Question

describe the plasmolytic method used to determine the osmotic pressure inside of Elodea sp. leaves in cell biology lab.

Answer 1

inside of Elodea method l describe the plasarelyhe used to determine the osmotic Pressure sp. Leaves in cell bielogy Lab. Pamela's method w Ebden sphoved: * Etool wash Elodeo Raves with top water leal with To do mount of on Elodia top water. en Prepare a wel this one place o drop of water towards end of the slide forceps used remove a small leaf from the tip of an Elidia plant and lay of Plat in the drop both Observe the leaf at 40 x and record seperately whatever the on Observation to 100x Porease the magnification serve and secever again wherever the brervations * Now Pud Elodeo leave in 5% salt sor and observe, (19 Remove the slide from the stage of the microscope (2) make the new slice of Elodea leave (3) Put 2 drops of the 5% salt solution on the neco stile at the left - edge of the cover sbp. (6) Tear off the a small peice of paper towel and place the tom edge on the Ahle at the right edge of the cover slip. The piece of towel should begin to Soak up water drawing the salt solation under the cover slip as it does so. (5) Put the slide to the microscope and observe the cells. 16) Make notes of the whol over observe under 4ox (2) change 9 increas the magnification to soon and record what ever obsese * Now Put Elodea leaves in 10% salt solution and obsere 0 Remove the least side of st sall solo & make meso slice of Elodea loves (3) Put 2 drops of 10 7 salt soluture on the new Glide at 16 Tear off the paper towel and put on slide it will soak up the water - crowing the sall solution ander the cover SV and does so lo put the slide to the nicerope and observe the slide make records what ever observe ender Gox magnification of microscope () Increase the magnification to loo x and record whatever observe * flushing out the salt solution - to Remove the slide from the stage of the microrope. (2) place 2-3 drops of top water on the shole at the cover itp. Observatims (3) Draw the water through using a sprall piece of paper towel. 14 Obuove the cells at 40x and 100x Record your observations. Remor the slide from the stage clean it and the cover slip and put "she away. a prepare sketches of a group of Elodeo cells under each set of condition get a flap water, 5% salt solution, amel sof soult solution) es label the magnification under which the plant alls are being observed (407, 200 xj o label the sketches to note the cell structure that you n be sure to note any changes in the cobur, size and shape and Entre of the cells. o 4 the coll dizi increasing is different medium than the osmotic mure also tocrease and if the cell & dize chrink in different meeting than the ormete Pressure will decrease Smalme de your skelches as accurale as pensil CamScanner to note the cell structure that you can identity. -