Homework Answers
Promoter is the sequence where RNA polymerase will bind. The transcription of the gene will take place from transcription start site to Terminator. The translation will take place from start codon to stop codon. Intron contains GU at 5' end and AG at 3' end.
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1. Diagram a eukaryotic gene containing...
5. A eukaryotic protein-encoding gene contains two introns and three exons: exon 1–intron 1–exon 2–intron 2–exon...
5. A eukaryotic protein-encoding gene contains two introns and three exons: exon 1–intron 1–exon 2–intron 2–exon 3. The 5ʹ splice site at the boundary between exon 2 and intron 2 has been eliminated by a small deletion in the gene. Describe how the pre-mRNA encoded by this mutant gene would be spliced. Indicate which introns and exons would be found in the mRNA after splicing occurs
Draw a Eukaryotic Gene Schematic Draw features of importance at the DNA level Transcription start site...
Draw a Eukaryotic Gene Schematic Draw features of importance at the DNA level Transcription start site +1 Promoter - as much detail as you can Gene start ATG and stop codons Transcription Regulatory Sequences such as activators/repressors and enhancers/insulators Draw features of importance at the pre-mRNA level Designate Introns and Exons Designate important Sequences to direct and regulate splicing three important sequences for the chemistry of splicing splicing regulatory sequences (ISS, ISE, ESE, ESS) Modifications at level of pre-mRNA UTRs,...
The diagram below shows a structure of an eukaryotic gene. Each pattern refers to a different...
The diagram below shows a structure of an eukaryotic gene. Each pattern refers to a different part of a gene. Using the pattern, label the diagram for the location of following: a. enhancer, b- promoter, c- transcription start, d- translation start: e- exon, i- intron, f- coading sequence, t1 -transcription stop, t2 -translation stop Put the corresponding letter on top of the pattern to show the location b. How pre-mRNA transcribed from this gene will look like? You may use...
Hint 1. How is a complete initiation complex formed? Drag the labels to their correct locations...
Hint 1. How is a complete initiation complex formed? Drag the labels to their correct locations in the diagram to describe the roles of the various transcription factors in the formation of a complete initiation complex Reset Help part of initial committed complex TFIIA IIB added during formation of minimal initiation complex sa cosci.ccccccca RNA polymerase II added during formation of complete initiation complex Submit Request Answer Hint 2. How is pre-mRNA processed into mature mRNA? Select the two statements...
Please answer 4 and 5? 4. Alpha amanitin is found in poisonous mushrooms and irreversibly binds...
Please answer 4 and 5?
4. Alpha amanitin is found in poisonous mushrooms and irreversibly binds RNA polymerase lI in eukaryotic cells. Why do people die when they eat poisonous mushrooms? (Stating they are poisonous is not an acceptable answer. You need to explain why.] 13 points] 5. Draw a diagram representing the structure of the human DABEARS gene. This gene has 4 exons. The complete precursor mRNA sequence is 2017 bp, [6 pts] a. First draw the gene indicating:...
Can someone please help with these two questions? Thank you. 4. Here is an RNA transcript...
Can someone please help with these two questions? Thank you.
4. Here is an RNA transcript freshly transcribed from a gene. It is immature, meaning that it has not been processed yet into a mature mRNA. a) If you could zoom in on this molecule, what two things would you observe at the molecular level that would indicate to you that it is RNA and not DNA? b) Which end of the molecule was the last bit to be transcribed,...
Need help with biochemistry please 3. RNA splicing. Eukaryotic mRNAs are frequently spliced before they are...
Need help with biochemistry please
3. RNA splicing. Eukaryotic mRNAs are frequently spliced before they are translated. Algae have the smallest known intron. The sequence of the algal pre-mRNA before splicing occurs is shown below. exon 1 intron exon 2 5-AUGGAAAUUAAGUACUAUAUUGAAUUUCAGGUUGAAGAUUUAGGAAUGG-3' A) What is the sequence of the mature mRNA after splicing occurs? 5'- -3' B) What is the sequence of the polypeptide after translation occurs? (N-terminus) (C-terminus) C) Identify the type of mutation in the following mutant pre-mRNAs. [In...
Genes in eukaryotes are often organized into exons and intrans, which require splicing to produce an...
Genes in eukaryotes are often organized into exons and intrans, which require splicing to produce an mRNA that can be translated. The gene organization is the order of the DNA segments that comprise the gene starting with the promoter, the first exon, the first intron, the second exon, and so on. The interspersed intrans can make gene identification difficult in eukaryotesparticularly in higher eukaryotes with many introns and alternative spliced mRNAs. Prediction of many genes and their organization has been...
Please explain the following question and answer all 29. The central dogma of molecular genetics is...
Please explain the following question and answer all
29. The central dogma of molecular genetics is that DNA encodes an mRNA, and mRNA allows proteins to be made. In the lecture on making cDNA libraries there was a statement that jokingly) said "central dogma be damned". What is it about making a cDNA library that goes against the central dogma? A. although a primer is required to make a cDNA, the primer is simply a long run of "T's", B....
Genetics Worksheet Week 3: Gene Regulation and Epigenetics 1. Duchenne muscular dystrophy is caused by a mutation in a gene that is 2.5 million nucleotides in length and encodes a protein called dyst...
Genetics Worksheet Week 3: Gene Regulation and Epigenetics 1. Duchenne muscular dystrophy is caused by a mutation in a gene that is 2.5 million nucleotides in length and encodes a protein called dystrophin. The dystrophin protein itself is 3684 amino acids in length. Calculate below the approximate size of the mRNA that encodes dystrophin. Approximately what percentage of the gene that encodes dystrophin is intron sequence? The human genome encodes a much greater variety and number of proteins than the...
Draw a Eukaryotic Gene Schematic Draw features of importance at the DNA level Transcription start site +1 Promoter - as much detail as you can Gene start ATG and stop codons Transcription Regulatory Sequences such as activators/repressors and enhancers/insulators Draw features of importance at the pre-mRNA level Designate Introns and Exons Designate important Sequences to direct and regulate splicing three important sequences for the chemistry of splicing splicing regulatory sequences (ISS, ISE, ESE, ESS) Modifications at level of pre-mRNA UTRs,...
The diagram below shows a structure of an eukaryotic gene. Each pattern refers to a different part of a gene. Using the pattern, label the diagram for the location of following: a. enhancer, b- promoter, c- transcription start, d- translation start: e- exon, i- intron, f- coading sequence, t1 -transcription stop, t2 -translation stop Put the corresponding letter on top of the pattern to show the location b. How pre-mRNA transcribed from this gene will look like? You may use...
Hint 1. How is a complete initiation complex formed? Drag the labels to their correct locations in the diagram to describe the roles of the various transcription factors in the formation of a complete initiation complex Reset Help part of initial committed complex TFIIA IIB added during formation of minimal initiation complex sa cosci.ccccccca RNA polymerase II added during formation of complete initiation complex Submit Request Answer Hint 2. How is pre-mRNA processed into mature mRNA? Select the two statements...
Please answer 4 and 5?
4. Alpha amanitin is found in poisonous mushrooms and irreversibly binds RNA polymerase lI in eukaryotic cells. Why do people die when they eat poisonous mushrooms? (Stating they are poisonous is not an acceptable answer. You need to explain why.] 13 points] 5. Draw a diagram representing the structure of the human DABEARS gene. This gene has 4 exons. The complete precursor mRNA sequence is 2017 bp, [6 pts] a. First draw the gene indicating:...
Can someone please help with these two questions? Thank you.
4. Here is an RNA transcript freshly transcribed from a gene. It is immature, meaning that it has not been processed yet into a mature mRNA. a) If you could zoom in on this molecule, what two things would you observe at the molecular level that would indicate to you that it is RNA and not DNA? b) Which end of the molecule was the last bit to be transcribed,...
Need help with biochemistry please
3. RNA splicing. Eukaryotic mRNAs are frequently spliced before they are translated. Algae have the smallest known intron. The sequence of the algal pre-mRNA before splicing occurs is shown below. exon 1 intron exon 2 5-AUGGAAAUUAAGUACUAUAUUGAAUUUCAGGUUGAAGAUUUAGGAAUGG-3' A) What is the sequence of the mature mRNA after splicing occurs? 5'- -3' B) What is the sequence of the polypeptide after translation occurs? (N-terminus) (C-terminus) C) Identify the type of mutation in the following mutant pre-mRNAs. [In...
Please explain the following question and answer all
29. The central dogma of molecular genetics is that DNA encodes an mRNA, and mRNA allows proteins to be made. In the lecture on making cDNA libraries there was a statement that jokingly) said "central dogma be damned". What is it about making a cDNA library that goes against the central dogma? A. although a primer is required to make a cDNA, the primer is simply a long run of "T's", B....
Genetics Worksheet Week 3: Gene Regulation and Epigenetics 1. Duchenne muscular dystrophy is caused by a mutation in a gene that is 2.5 million nucleotides in length and encodes a protein called dystrophin. The dystrophin protein itself is 3684 amino acids in length. Calculate below the approximate size of the mRNA that encodes dystrophin. Approximately what percentage of the gene that encodes dystrophin is intron sequence? The human genome encodes a much greater variety and number of proteins than the...
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