Question

Draw a Eukaryotic Gene Schematic Draw features of importance at the DNA level Transcription start site +1 Promoter - as much detail as you can Gene start ATG and stop codons Transcription Regulatory Sequences such as activators/repressors and enhancers/insulators Draw features of importance at the pre-mRNA level Designate Introns and Exons Designate important Sequences to direct and regulate splicing three important sequences for the chemistry of splicing splicing regulatory sequences (ISS, ISE, ESE, ESS) Modifications at level of pre-mRNA UTRs, start codon and stop codon Draw features of importance at the spliced mRNA level Modifications at level of processed mRNA UTRs, start codon and stop codon Some type of translational regulation Narrative (2) Write a few sentence narrative about how your gene expression is regulated at each step.

Answer 1

Regulatory sequence OPEN READING FRAME CORE I ISTART STOP, S'UTRI - Terminator -45 5 UTR Enhances Silences 'Enhancer) 'PROMOTER I Silencer Regulatory sequence Eukaryotic Gene Sequence.

A texon exon exon lexon pre- MRNA (U2 sn RNP Hiss ITBP (U2AF65 (V2AF35( IPP track - AGTESEN ESS Exons and Introns of pre-mRNA with ESELESS - Exonic splicing sequences ISE ASS - inhronic splicing sequences and splicing factors U1 sn RNP, U2 SOR NP , U2A565, U2 AF 35 and positive a and negative splicing regulatory proking, ne

Intron Poly (A) tail slop ap 50 5' UR Start Exon short stop Exon AAAA - - -3 start stop Exon one B' UTR RNA splicing start 5 on from 1 Exon . Exon AAAAA --- 3 coyo 3 UTR 5' UTR

· Eukaryotic promoters are the sequences that are used for the initiation of transcription. The promoters consists of core promoter and upstream control elements (UCE) that are located upstream of the core promoter.

· Core promoter is the region of -45 to +20 nucleotides with a start point at +1 nucleotide.

· Different RNA polymerase uses different promoters for their activity.

Narrative on gene expression regulation:

· Enhancers and repressors are the regulating elements that regulate the activity of promoters in eukaryotes.

· Enhancers and repressors serve as binding sites for the specific regulatory proteins

· The mRNA molecule is stabilized by the cap and poly A tail that prevents mRNA from the action of exonucleases

· DNA binding regulatory proteins involved in the regulation of transcription of DNA.