Question

Now it's your turn to construct a dilution scheme to achieve a countable plate. You start with a stock culture of 5.4 x 10^8 cells/mL. Draw a dilution scheme that will produce a countable plate (<300 colonies). Show how much is transferred at each step, how much media is in each flask or tube, and how much you will plate. Show how many colonies you expect on your final plate. Once you have constructed your scheme, answer this question: Is your scheme economical in its use of broth media, i.e. could you produce the same result and use less media?

Answer 1

• We need dilute the stock with dilution of 10- and must O plate o.1 ml of final diluted tubes than we can expect countable Colony. Scheme: Total CHU =( 5.4 %108 X10-0 X 0.1) - 54 CFU (300CFU. Calter dilution) and Oill som O.lm & rolm o .lmper 10.9m1 Co.ami Co.ami foname I joiame 10.am) 10-4 (0-5 "54 CFU 10-2 10-3 lang 10-1 . Coulmi

> pes, my scheme Es economical and we meed very less amount of mudrien't media (broth) qe ubto 6ml of broth but we can also get result by using less media but on that case Calculation culll be difficult sind there will higher Chance of error, hence we must stick to our scheme