Question

You have created a YAC and want to place a set of genes that need to be expressed near one of the telomeres.

1. What will you need to consider in your YAC re-engineering design to ensure that these genes are expressible?
2. Would this same approach work to express engineered genes in an Xi chromosome? Provide a rationale for your answer.

Answer 1

Yeast artificial chromosome- All autonomous Plasmid Vectors maintained in yeast as circular DNA malecule which possess yeast centromere. The end of all en yeast chromosome yeast chromosome like thase af all other linear eukaryotic chroma eukaryotic chromosome have unique structure known telomere. Telomere structure have evolve as a device to Preseved integrity of the end of malecule of DNA, Replication mechanism are started at tilomenic region. without template. of yeast cloning tilomere into a YRp such vector are known as yeast artificial chromosome. (1) CENT Cloning site Amp HURA 3 PYAC Vector TEL BamHI TEL Target DNA Т? Ако се TURA D Target DNA fragment Ligase. URA TEL Reassemble chromosomes in YAC.

^ chromosome are the regulation af chromosome that are inactivate the epigenetic regulation of e athey gene in the coat the chromosome an is and induces silencing af although some gene avide silencing in most type at they escape XCI only in the specific context, Genetic engineering chromosome are regulated. the gene expression for the Chromosome, that are ekigenetic regulation of the chromosome.