Question

TSI Triple Sugar Iron Agar Data Sheet The enterics, specifically coliforms, will show what result in the TSI? What is reversion and what organisms showed reversion in the TSI? What does a black color in the butt of the slant indicate in the TSI? What is the pH indicator in the TSI and what is the purpose of the pH indicator? Do all organisms use the sugars in the TSI? What organisms do not utilize the sugars and what is the result in the TSI? What is the function of the reducible sulfur in the media? What is the function of the H, Sindicator in the media? What organism shows a positive result for protein catabolism? What organism shows a positive result for cystein catabolism and sulfur reduction? Can organisms that ferment glucose also ferment lactose? Why or why not?

Answer 1

Triple sugar iron agar (
Question The enterics, specifically coliturms, which will show what result in TSIZ wer P Alkaline slant (Red) Answer A) No carbohydrate fermenter - ho Alkaline butt (Red) B) Only glucose fermenter - Alkaline slant (Red) - L Acid butt (Yellow) o Lactose I sucane fermenter so Acid slant (Yellow) - 4 Acid butt (Yellow) D HAS producing organism → Blackening of butt E Gas producing organisms Bubble or erack in B CO2 or Hydrogen ) agar OVOS NO cose Question is What is reversion and what organisms show reversion en TSI ? Answer Organisms which terment glucose and pooduce acid will change colour from red to yellow initially, But after a few hours, since glucoge es present en very small concentration (0.1%), these organisms start using peptune and amino acids for their energy , now producing alkaline medium and thus reverting colour back to red. This phenomenon is termed as reversion. Salmonella and Shigella show this phenomenon.

Question - What does a black colour in the butt of slant. indicate in ISI? Answer: Black colour in butt indicate sulfide producing bacteria Hydrogen sulfide produced by bacteria reacts with Ferrous sulfate to form ferrous sulfide which gives black colour. Salmonella, Proteus, Citrobacter are examples of sulfide producing bacteria. Question what is pH indicator in TSI and what is the purpose of pH indicator ? Answert a Phenol red is the indicator used in TSI. When bacteria ferment any of the three sugars present in the medium, it will lead to production of acid Acidic medium will change colour al phenol red from red to yellow this it acts au pH indicator a

Question - Do all organisms used the sugars en TSI ? Answerin No, non-fermenters do not use sugar in 7579 Questions What organism do not utilize the sugar and what is the result in TSIE Answer:. Preudomonas. I TSI slant is red and butt - usred without any gas or H₂S production, Questions What is the function of reducible sulfur - en media? Answer in Hydrogen sulphide producing bacteria will reduce ferrous sulphate to form ferrous sulphide giving black colour to butt.
TSI) is a differential medium used to differentiate various members of Enterobactericae or enteric based on their properties. It contains a) agar b) phenol Red c) 1% lactose d) 1%sucrose e) 0.1%glucose f) sodium thiosulfate g) ferrous sulfate