Question

2a Which reactions require ATP? Which reactions produce ATP? What is the net yield of ATP for every mole of glucose that enters the glycolysis pathway? 2b What are AG' and AG' for the glycolysis pathway? Is this pathway catabolic or anabolic? 2c Which step is an illustration of a reverse aldol condensation? 2d. Define substrate level phosphorylation. Which of these compounds in the glycolysis pathway have a large phosphate group transfer potential? 2e. Which steps are examples of redox reactions? What compounds are oxidized and which ones are reduced?

Answer 1

2.a) ATP Requiring steps-

Glucose ---------- Glucose-6-Phosphate (By Hexokinase)

Fructose-6 Phosphate----------Fructose1,6 Bisphosphate (by Phosphofructokinase)

ATP Producing steps-

1,3 Bisphosphoglycerate-------------3 Phosphoglycerate (Phosphoglycerate Kinase)

Phosphoenol pyruvate-------------- Pyruvik acid ( By pyruvate kinase)

The total yield of ATP from one molecule of Glucose is 2 (total production 4 , total consumption 2).

2.b) In Total as a whole DelG0 is -35.87 KJ/Mol

In Total as a whole DelG0 is -76 KJ/Mol

Glycolysis is Catabolic Pathway ( although many anabolic pathways are rlient on glycolysis)

2.c) the step of onversion of Fructose1,6 bisphosphate to glyceraldehyde 3 phosphate and dyhydroxy acetone phosphate is an example of reverese aldol condensation ( catalysed by aldolase)

2.d) Rather than the formation from ADP and Pi if ATP is formed by the transfer of a phosphate group from a phosphorylated intermidiate to ADP and ATP is generated, then its called substrate level phosphorylation.

1,3 Bisphosphoglycerate and Phosphoenol pyruvate both has a high phosphate donation potential.

glyceraldehyde 3 phosphate to 13 bpg step i a redox step in which glyceraldehyde 3 phosphate is oxydised.