Question

9. The image below shows a hemocytometer with ovals drawn to simulate cells. The cells were stained with trypan blue by adding staining solution to cells in a 1:1 ratio. a. Calculate the cell density of the original suspension in cells per ml. b. What volume, in ml, of the original cell suspension would you add to a 35-mm dish? See the link below for the number of cells to add: https://www.thermofisher.com/us/en/home/references/gibco-cell- culture-basics/cell-culture-protocols/cell-culture-useful- numbers.html Show your calculations. 18 lott IO TTT ako

Answer 1

(a) Cell density of the original suspension in cells per ml= Total number of cells /4*105

=3+4+5+7/4*105=4.75*105cells/ml

(b) Volume in ml for 35mm dish=0.3*106/4.75*105=30/4.75=6.32 ml

[Seeding density for 35mm dish =0.3*106]