Homework Answers
AK400 strain has Lac+ gene which metabolize lactose in the
medium. So in MacConkey agar
it form red colonies.
AK403 is Lac- strain which not able to metabolize lactose in the
medium. So in MacConkey agar
it form white colonies.
When AK400 Lac+ gene is transfer to AK403 strain, AK403 also now
has a capacity
to metabolize lactose and forma a Red colonies in the medium.
The only difference we can see in the AK403 after conjugation is
it contain both white and red colonies
because not all AK403 undergoes conjugation so still some cells
have Lac- gene.
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6. Since AK400 harbours a Lac+ plasmid and is, thus, able to utilize lactose, how is...
Lactose (or rather, its derivative allolactose) can lift the Lac Repressor protein from the Operator site...
Lactose (or rather, its derivative allolactose) can lift the Lac Repressor protein from the Operator site in DNA, thus allowing for expression of the genes coded by the Lac Operon. However, lactose cannot cross the bacterial cell membrane without the help of the Permease protein channel, which is coded by the lacY gene in the Lac Operon. So, it appears that we have a case of "what's first, the chicken or the egg?" - how can lactose enter the cell...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
A. Which strain is the wild-type E.coli? Explain how you know this. B. Which strain contains...
A. Which strain is the wild-type E.coli? Explain how you know
this.
B. Which strain contains the nonsense mutation in the CRP
protein gene? Explain how you know this.
C. Which strain contains the deletion mutation in the lac operon
operator sequence? Explain how you know this.
knowledge about the lac operon system AND knowledge Problem set about the Lac operon (these problems require knowledge about the lac operon system AND about consequences of mutations) You are working with three...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the F' plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the F' segment...
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon...
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon is regulated. The descriptions of some of the mutants are listed. For each E. coli mutant determine if the lac operon would be on or off in a lactose-only environment. laci mutant: This mutant produces a super repressor that is not inactivated by allolactose. It will still stick. Operater will be off, lacO mutant: This mutant has an altered operator sequence so that the...
You are asked to develop a demonstration to show how the lac operon works. You decide...
You are asked to develop a demonstration to show how the
lac operon works. You decide to use X-gal and IPTG to
determine if the enzyme ?-galactosidase is active. X-gal is a
lactose analog that turns blue when metabolized by ?-galactosidase,
but it does not induce the lac operon. IPTG is an inducer
of the lac operon, but is not metabolized by ?-galactosidase.
a. (2pts) Which of the following would you expect to bind to
?-galactosidase. Circle all that apply....
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome...
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome of recipient Figure 8 Double- Cel stranded Cytoplasmic ll ONA Integrated DNA Treat with electric current Recipient cell Fragments of donor DNA Transformed cell Phage Figure 9 Figure 10 Phage DNA enters the cell Bacterial host #1 앗 O E. coli F plasmid Gov Integration of F plasmid Bacterial DNA -Bacterial host #2 85 Replaced host DNA Bacterial DNA I Which diagram(s) depict conjugation?...
for 1-5 define those The Lac operon is an inducible set of genes found in bacteria...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
Please answer these four questions. 6C. A type of plasmid used frequently as a vector in...
Please answer these four questions.
6C. A type of plasmid used frequently as a vector in recombinant DNA procedures carries gene markers for resistance to tetracycline (Tet') and to ampicillin (Amp). In one experiment, such plasmids were exposed to a restriction enzyme that cuts within the Amp' gene but leaves the Tet' gene intact. Plasmids exposed to the enzyme were next mixed with genomic restriction fragments from a cloned library of mouse DNA. The aim was to produce and detect...
As a student project, you have isolated six new mutant strains of E. coli with altered...
As a student project, you have isolated six new mutant strains
of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
A. Which strain is the wild-type E.coli? Explain how you know
this.
B. Which strain contains the nonsense mutation in the CRP
protein gene? Explain how you know this.
C. Which strain contains the deletion mutation in the lac operon
operator sequence? Explain how you know this.
knowledge about the lac operon system AND knowledge Problem set about the Lac operon (these problems require knowledge about the lac operon system AND about consequences of mutations) You are working with three...
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon is regulated. The descriptions of some of the mutants are listed. For each E. coli mutant determine if the lac operon would be on or off in a lactose-only environment. laci mutant: This mutant produces a super repressor that is not inactivated by allolactose. It will still stick. Operater will be off, lacO mutant: This mutant has an altered operator sequence so that the...
You are asked to develop a demonstration to show how the
lac operon works. You decide to use X-gal and IPTG to
determine if the enzyme ?-galactosidase is active. X-gal is a
lactose analog that turns blue when metabolized by ?-galactosidase,
but it does not induce the lac operon. IPTG is an inducer
of the lac operon, but is not metabolized by ?-galactosidase.
a. (2pts) Which of the following would you expect to bind to
?-galactosidase. Circle all that apply....
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome of recipient Figure 8 Double- Cel stranded Cytoplasmic ll ONA Integrated DNA Treat with electric current Recipient cell Fragments of donor DNA Transformed cell Phage Figure 9 Figure 10 Phage DNA enters the cell Bacterial host #1 앗 O E. coli F plasmid Gov Integration of F plasmid Bacterial DNA -Bacterial host #2 85 Replaced host DNA Bacterial DNA I Which diagram(s) depict conjugation?...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
Please answer these four questions.
6C. A type of plasmid used frequently as a vector in recombinant DNA procedures carries gene markers for resistance to tetracycline (Tet') and to ampicillin (Amp). In one experiment, such plasmids were exposed to a restriction enzyme that cuts within the Amp' gene but leaves the Tet' gene intact. Plasmids exposed to the enzyme were next mixed with genomic restriction fragments from a cloned library of mouse DNA. The aim was to produce and detect...
As a student project, you have isolated six new mutant strains
of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
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