![29.12 pts] Three genes (lacZ lacY and lacA) are all transcribed onto a single mRNA by RNA polymerase utilizing eherefore, the](http://img.homeworklib.com/questions/b86f69d0-bf1e-11ea-9672-57993bc8f00e.png?x-oss-process=image/resize,w_560)
Homework Answers
1) figure 6 and 7 depicts conjugation, conjugation is the process by which genetic material is transferred from one bacteria to another via sex pili, so the answer is E) figures 6 and 7
2) after mating of 2 cells in figure 7, the plasmid is integrated into host DNA so both cells become Hfr so the answer is B) are both Hfr.
3) figure 8 shows transformation using the electroporation method so the answer is B) transformation
4) Figure 9 depicts the transduction of bacteria with a virus so the answer is D) transduction
5) in figure 10 shows the integration of F plasmid to bacterial genome so that the cell becomes Hfr so the answer is A) conversion of an F+ cell to an Hfr cell.
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Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome...
Illustrate a conjugation event with an F’ cell that is lacIS lacP+lacO+lacZ- lacY-on the bacterial chromosome...
Illustrate a conjugation event with an F’ cell that is lacIS lacP+lacO+lacZ- lacY-on the bacterial chromosome and lacI- lacP+ lacOC lacZ+ lacY+ on an F plasmid with an F-cell that is lacI+ lacP+ lacO+ lacZ+ lacY-. Draw both cells before the conjugation event, as well as the recipient cell after conjugation occurs. Underneath all three cells, note whether the production of permease and beta-galactosidase would be high, basal, or none, and describe HOW the proteins and DNA sequences interact to...
25. You take your lac/ strain and transform in two F' plasmids to conduct a complementation...
25. You take your lac/ strain and transform in two F' plasmids to conduct a complementation test. The first F' plasmid contains the entire lac operon from a strain that is lacz, while the second F' plasmid contains the entire lac operon from a strain that is lacY. You test the ability of the transformed bacteria to grow on media where the sole carbon source is either glucose or lactose, and obtain the following results: Lac t mutant compl lacZ...
QUESTION 34 In Hfr strains, the F plasmid is attached to a phage attached to the...
QUESTION 34 In Hfr strains, the F plasmid is attached to a phage attached to the cell wall free in the cytoplasm integrated into the chromosome QUESTION 35 Which of the following is NOT a molecule found during DNA replication? mRNA DNA polymerase 1 helicase ORNA primer Click Save and Submit to save and submit. Click Save All Answers to save all answers. ch.16 PT AP n...ot a ch. 18 PPT AP noppbe ch 17 PPT AP n. e @...
Background: Several genetic regions are needed for lactose metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene), lacY(permease gene), lacA(transacetylase...
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
for 1-5 define those The Lac operon is an inducible set of genes found in bacteria...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and a live bacterial cell is: a. conjugation b. transduction c. transversion d. transformation 2. An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+...
Which The figure represents the trp (A-C) and the lac (D-G) operons. A and D show...
Which
The figure represents the trp (A-C) and the lac (D-G) operons. A and D show the operons without any proteins bound to them. The others have one or more proteins bound to them. Use the figures your notes to answer the next four questions. OK? = este lace laco acz | lacy = D = trpP tro trpe trpo trpetrpctra EA lacp Lac Rep- pressor lacz lacy RNAP holo enzyme trpo tro trpe trpe treo trpD trpB trpe trc...
forme este New York State Depart Request for COMID-19 * Take Test LECTURE EX G Which...
forme este New York State Depart Request for COMID-19 * Take Test LECTURE EX G Which b bhosted.cuny.edu/webapps/assessment/take launch ofcourse assessment id. 1399019 1course de 16 € > C Remaining Time 39 minutes, 18 seconds Question Completion Status QUESTION 39 Two human diseases caused by viruses are O polio and bovine spongiform encephalopathy smallpox and polio O malaria and tuberculosis smallpox and bovine spongiform encephalopathy QUESTION 40 A process that requires a virus to transfer DNA between two bacterial cells...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the follow...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the...
A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the structural genes with the reporter gene green fluorescent protein (GFP). The repressor allele cannot bind lactose, and the OC allele cannot be bound by the repressor. Given the following genotypes and growth media that does not contain glucose, indicate in the table below whether you expect to see b-galactosidase activity and glowing green colonies of E. coli by selecting Yes or No. growth media...
25. You take your lac/ strain and transform in two F' plasmids to conduct a complementation test. The first F' plasmid contains the entire lac operon from a strain that is lacz, while the second F' plasmid contains the entire lac operon from a strain that is lacY. You test the ability of the transformed bacteria to grow on media where the sole carbon source is either glucose or lactose, and obtain the following results: Lac t mutant compl lacZ...
QUESTION 34 In Hfr strains, the F plasmid is attached to a phage attached to the cell wall free in the cytoplasm integrated into the chromosome QUESTION 35 Which of the following is NOT a molecule found during DNA replication? mRNA DNA polymerase 1 helicase ORNA primer Click Save and Submit to save and submit. Click Save All Answers to save all answers. ch.16 PT AP n...ot a ch. 18 PPT AP noppbe ch 17 PPT AP n. e @...
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
Which
The figure represents the trp (A-C) and the lac (D-G) operons. A and D show the operons without any proteins bound to them. The others have one or more proteins bound to them. Use the figures your notes to answer the next four questions. OK? = este lace laco acz | lacy = D = trpP tro trpe trpo trpetrpctra EA lacp Lac Rep- pressor lacz lacy RNAP holo enzyme trpo tro trpe trpe treo trpD trpB trpe trc...
forme este New York State Depart Request for COMID-19 * Take Test LECTURE EX G Which b bhosted.cuny.edu/webapps/assessment/take launch ofcourse assessment id. 1399019 1course de 16 € > C Remaining Time 39 minutes, 18 seconds Question Completion Status QUESTION 39 Two human diseases caused by viruses are O polio and bovine spongiform encephalopathy smallpox and polio O malaria and tuberculosis smallpox and bovine spongiform encephalopathy QUESTION 40 A process that requires a virus to transfer DNA between two bacterial cells...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the structural genes with the reporter gene green fluorescent protein (GFP). The repressor allele cannot bind lactose, and the OC allele cannot be bound by the repressor. Given the following genotypes and growth media that does not contain glucose, indicate in the table below whether you expect to see b-galactosidase activity and glowing green colonies of E. coli by selecting Yes or No. growth media...
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