Question

A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the structural genes with the reporter gene green fluorescent protein (GFP). The repressor allele cannot bind lactose, and the OC allele cannot be bound by the repressor. Given the following genotypes and growth media that does not contain glucose, indicate in the table below whether you expect to see b-galactosidase activity and glowing green colonies of E. coli by selecting Yes or No. growth media GFP Genotype a) I+O+ ZH F' I+O+ GFP+ b-gal Click for List + lactose Click for List - lactose Click for List Click for List b) /+ OC ZH F' 0+GFP+ + lactose Click for List Click for List - lactose Click for List Click for List Multiple T/F. c) The lacZ gene and the human LCT gene are orthologs that are both regulated in response to lactose in the environment. Click for List Lactase persistence in adult humans: d) is caused by a nonsynonymous mutation that alters the activity of the LCT enzyme. Click for List e) is caused by recent positive selection at the LCT locus acting on different regulatory mutations present in African and northern European populations. Click for List f) What is in common between the GAL, lac and trp gene regulatory systems? Coordinated regulation of enzymes to meet cellular metabolic needs in the context of the environment. Allosteric regulation by small molecules that are either anabolized or catabolized by the enzymes whose expression is being regulated. Inducible gene expression that conserves energy for the cell. Coupled transcription and translation. Chromatin regulation to coordinate structural gene expression. trans-acting proteins that bind cis-acting DNA sequences.