Question

utiple Choice. Circle the correct answer choice (1 point each) which in or which ingredient(s) are found in glucose broth? Water Sodium Chloride Glucose Peptone Magnesium sulfate Dipotassium phosphate Beef extract Agar Ammonia dihydrogen phosphate 2. Which ingredient(s) are found in nutrient broth? Water Sodium Chloride Glucose Peptone Magnesium sulfate Dipotassium phosphate Beef extract Agar Ammonia dihydrogen phosphate 3. Which ingredients in the nutrient agar make it a complex media? Water Sodium Chloride Glucose Peptone Magnesium sulfate Dipotassium phosphate Beef extract Agar Ammonia dihydrogen phosphate 4. How can you tell that you had different microbe growing on your Petri dish? a. All the colonies look the same. b. Several colonies look different. c. There are always many microbes growing on the agar. d. I can't determine this. 5. What is not a benefit of using agar? a. It is a solidifying agent used for microbial growth. b. It can be metabolized by microbes. c. It can be liquified easily to pour agar plates. d. It can be used to prepare solid media to detect the growth of different microorganism,

Answer 1

1) Water

Sodium Chloride

Glucose

Peptone

Reason : No explanation required. These are the ingredients used in preparation of Glucose Broth. Agar is used, but not included since it is in broth form.

2) Water

Sodium Chloride

Peptone

Beef Extract

Reason : No explanation required. These are the ingredients used in preparation of Nutrient Broth. Agar is used, but not included since it is in broth form.

3) Peptone

Beef Extract

Agar

Reason : Complex media is the media whose exact composition is not known due to variable compositions. Peptone, Beef Extract and Agar are a mixture of several biomolecules, and their composition is never fixed. The constituents of Agar, Peptone and Beef Extract will always vary when their source is different, as they are not synthesised artificially. Since they are derived from natural sources only, hence their variable composition makes the media complex.

4) The correct answer is option b.

Reason : Multiple microorganisms usually grow in colonies, which differ from each other in terms of colour, size and morphology. To check the growth of different microbes, we'll visualise different types of colonies on the petri dish. Different colored and shaped colonies will represent different types of microorganisms.

5) The correct answer is option b

Reason : The biggest factor promoting the use of agar instead of other gelling agents is the fact that Agar is basically indigestible by most of the microorganisms. Since Agar cannot be metabolized by microbes, so the microbial growth won't be able to affect the integrity of the gel.The other three options are the benefits associated with using Agar.

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