Question

Green Fluorescent Protein Part III: Restriction Digests and Gel Electrophoresis Plasmid Tube # Water | 10X Buffer Restriction Enzyme(s) DNA 6 ul . 10 pl 10 ul 10 ul 10 ul 6 ul 6 ul 6μ 2 ul 2 ul 2 ul 2 ul 2 pl Nde! 2 ul Pst. 2 ul [Enzyme A] (single cut choice) 2 ul Pst | + 2 ul [Enzyme Al 2 pl [Enzyme B] + 2 pl [Enzyme C] (two enzymes to cut out GFP gene) no restriction enzyme - uncut plasmid 10 ul 6 μι 2 ul 10 ul 8 ul 2 ul 1-1) Tub e # Restriction Enzyme(s) Number of Fragments Fragment Lengths (bp) Nde Pst Enzyme A Pst | + Enzyme A Enzyme B+ Enzyme C None 1-2) Provide a BRIEF paragraph describing how your results compare with your predictions (from the table in the previous question).

2. Tetrads form during _______.

prophase of mitosis

metaphase of mitosis

prophase of meiosis I

metaphase of meiosis I

telophase of meiosis I

prophase of meiosis II

metaphase of meiosis II

anaphase of meiosis II

telophase of meiosis II

anaphase of meiosis

3. A normal individual who is a carrier for an x-linked trait like hemophilia ___.

SELECT ALL THAT APPLY.

is heterozygous for the recessive condition.

shows the dominant phenotype.

can have daughters who have the gene.

is always female.

Answer 1

Ans1.) Bivalents or tetrads formation takes place in prophase of meiosis 1 . The leptotene stage begins with the condensation of chromatin fibers into long, threadlike structures, similar to what occurs at the beginning of mitosis. At zygotene, continued condensation makes individual chromosomes distinguishable, and homologous chromosomes become closely paired with each other via the process of synapsis, forming bivalents.

Ans2.)Since the disease is X-linked so males can never serve as carrier, they will always show the diseased phenotype because of having one X-ray chromosome.

So a normal individual who is a carrier for the X-linked trait  is always a women, can have daughters who have the gene, is hetrozygous for the recessive condition, show as the dominant ( sufferer) phenotype in males only carrying that allele.

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