Homework Answers
![Velocity in absence of inhibitor VE Vmax [s] [s]+ km 2 Ve 300x109 x 2x105 2x105 + 6.7 8109 6.9816-4 V = 8.69 X 10 - 9 moles /](http://img.homeworklib.com/questions/1e41b410-e81f-11eb-a785-f74370b8a38e.png?x-oss-process=image/resize,w_560)
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Q1: A marine microorganism contains an enzyme that hydrolyzes glucose-6- sulfate (S). The assay is based...
1. An enzyme has a km of 4.7 X 105M. If the Vmax of the preparation...
1. An enzyme has a km of 4.7 X 105M. If the Vmax of the preparation is 224M/min, what velocity would be observed in the presence of 2 X 10^M substrate and 5 X10+M a competitive inhibitor. Ki is 3 X 10^M. What is the degree of inhibition? (10pts)
An enzyme binds to a competitive inhibitor with Kd = 1.2 × 10-6 M at pH 7.0 and 25°C.
An enzyme binds to a competitive inhibitor with Kd = 1.2 × 10-6 M at pH 7.0 and 25°C.(a)At what inhibitor concentration will 75% of the enzyme be bound to the inhibitor if there is no substrate present? (b) This enzyme has a Km of 4.0 × 10-5 M and a Vmax of 50 μM/s. At a substrate concentration of 3.0 × 10-4 M, calculate (i) the velocity of reaction in the presence of the inhibitor at 4.8 x 10-5 M (ii) the degree of...
A biochemist studies the properties of a metabolic enzyme she has just isolated. She obtains kinetic data in the presen...
A biochemist studies the properties of a metabolic enzyme she
has just isolated. She obtains kinetic data in the presence and in
the absence of two different inhibitors (A and B). The identity of
the inhibitors is unknown but we know that one of these is an
substrate analog while the other is an alkylating agent.
From the plot above determine:
a) Km and Vmax of the enzyme
b) which inhibitor is the substrate analog? Which is the alkylating
agent?...
Please help me with these two problems. I will rate the answers. 1. The HIV-1 protease,...
Please help me with these two problems. I will rate the
answers.
1. The HIV-1 protease, an important enzyme in the life cycle of the human immunodeficiency virus-1 (HIV-1), is a good drug target for the treatment of HIV and AIDS. A protein produced by the virus, p6*, is an HIV-1 protease inhibitor. The activity of the HIV-1 protease was measured in the presence and absence of p6* using an assay involving an artificial substrate, as shown below. NH Lys...
Can anyone help with parts D and E? Ibuprofen [a-methyl-4-(isobutyl)phenylacetic acid; Mr 206] reversibly inhibits cyclo-oxygenase...
Can anyone help with parts D and E?
Ibuprofen [a-methyl-4-(isobutyl)phenylacetic acid; Mr 206] reversibly inhibits cyclo-oxygenase (COX-2), a Michaelis enzyme that converts arachidonate to prostaglandin G2 (PPG2) thereby preventing the fever, inflammation and pain induced by prostaglandins. The following table shows a kinetic analysis of COX-2 in the absence and presence of 10 mg/ml Ibuprofen [Arachidonate] Vo mM/min w/o Ibuprofen Vo, mM/min w/ Ibuprofen mM 0.5 23.5 16.67 25.25 30.49 1.0 32.2 1.5 36.9 2.5 41.8 37.04 38.91 3.5 44.0...
2. Lactose is a disaccharide found in milk. Many adults throughout the world get sick from drinking milk because they cannot digest lactose. Lactose intolerance varies markedly among various human pop...
2. Lactose is a disaccharide found in milk. Many adults throughout the world get sick from drinking milk because they cannot digest lactose. Lactose intolerance varies markedly among various human populations. For example, only about 3% of people of Danish descent are lactose intolerant, compared with 97% of people of Thai descent. When someone who is lactose intolerant ingests milk, the lactose accumulates in the lumen of the small intestine because there is no mechanism for uptake of the disaccharide....
a. what are the values of Vmax and Km in the abscence if the inhibitor what...
a. what are the values of Vmax and Km in the abscence if the
inhibitor what are the values of Vmax and Km in the presence of the
inhibitor?
b. what type of inhibition is it?
c. what is the dissociation constant (Ki) of the
inhibition?
***d. graph a linear scatter plot including equation.
Homework (CHE 407) The initial velocity for an enzyme-catalyzed reaction is measured at various initial substrate concentration [S]o, in the absence and in the presence of...
Based on the document below, 1. Describe the hypothesis Chaudhuri et al ids attempting to evaluate;...
Based on the document below,
1. Describe the hypothesis Chaudhuri et al ids attempting to
evaluate; in other words, what is the goal of this paper? Why is he
writing it?
2. Does the data presented in the paper support the hypothesis
stated in the introduction? Explain.
3.According to Chaudhuri, what is the potential role of thew
alkaline phosphatase in the cleanup of industrial waste.
CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...
1. An enzyme has a km of 4.7 X 105M. If the Vmax of the preparation is 224M/min, what velocity would be observed in the presence of 2 X 10^M substrate and 5 X10+M a competitive inhibitor. Ki is 3 X 10^M. What is the degree of inhibition? (10pts)
A biochemist studies the properties of a metabolic enzyme she
has just isolated. She obtains kinetic data in the presence and in
the absence of two different inhibitors (A and B). The identity of
the inhibitors is unknown but we know that one of these is an
substrate analog while the other is an alkylating agent.
From the plot above determine:
a) Km and Vmax of the enzyme
b) which inhibitor is the substrate analog? Which is the alkylating
agent?...
Please help me with these two problems. I will rate the
answers.
1. The HIV-1 protease, an important enzyme in the life cycle of the human immunodeficiency virus-1 (HIV-1), is a good drug target for the treatment of HIV and AIDS. A protein produced by the virus, p6*, is an HIV-1 protease inhibitor. The activity of the HIV-1 protease was measured in the presence and absence of p6* using an assay involving an artificial substrate, as shown below. NH Lys...
Can anyone help with parts D and E?
Ibuprofen [a-methyl-4-(isobutyl)phenylacetic acid; Mr 206] reversibly inhibits cyclo-oxygenase (COX-2), a Michaelis enzyme that converts arachidonate to prostaglandin G2 (PPG2) thereby preventing the fever, inflammation and pain induced by prostaglandins. The following table shows a kinetic analysis of COX-2 in the absence and presence of 10 mg/ml Ibuprofen [Arachidonate] Vo mM/min w/o Ibuprofen Vo, mM/min w/ Ibuprofen mM 0.5 23.5 16.67 25.25 30.49 1.0 32.2 1.5 36.9 2.5 41.8 37.04 38.91 3.5 44.0...
a. what are the values of Vmax and Km in the abscence if the
inhibitor what are the values of Vmax and Km in the presence of the
inhibitor?
b. what type of inhibition is it?
c. what is the dissociation constant (Ki) of the
inhibition?
***d. graph a linear scatter plot including equation.
Homework (CHE 407) The initial velocity for an enzyme-catalyzed reaction is measured at various initial substrate concentration [S]o, in the absence and in the presence of...
Based on the document below,
1. Describe the hypothesis Chaudhuri et al ids attempting to
evaluate; in other words, what is the goal of this paper? Why is he
writing it?
2. Does the data presented in the paper support the hypothesis
stated in the introduction? Explain.
3.According to Chaudhuri, what is the potential role of thew
alkaline phosphatase in the cleanup of industrial waste.
CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...
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