Question

11. Determine the color and the absorbance of the light and dark cuvettes at time. a. Be quick when performing these steps; b. Mix/invert/wipe and insert each cuvette into the Smer-20 to determine absorbance at 550 nm (Asso) [make sure you have pre-blanked spectrophotometer). c. Determine the color/pH by comparing the color of the Co, indicator in your cuvette to the provided Indicator Color Guide. d. Start keeping track of time. e. Immediately re-cover the dark cuvette and position the cuvettes within 15-25 cm of the light source after taking a reading. The cuvettes must remain in the light or the dark as much as possible. 12. Begin taking readings every 5 min, making sure to blank in advance and thoroughly mix the CO2 indicator in the cuvettes prior to measuring and determining the color and pH. a. Record your color/pH information and your absorbance data in Table 1. Results Table 1 Mort Olkaline Indicator color/pH, and absorbance of light and dark cuvette More Acidic. X Time in minutes Light Dark Asso & Indicator color/pH Asso & Indicator color/pH -79 -0.0087.9 0.80 8 -0.0081 7.9 10 0.180 8.2.1-0.0.71 7 .7 0.70 8.6 -0.0907 20 1.90 0.7 -0.0007 0.7 -0.074 30 0.750 18.4 -0.098 7.3 1 708.4-0.100 1.190. 9.1-0.101 PILIPP: SEM 35 40 45 Analysis and Discussion The goal of this analysis is to determine the rates of photosynthesis and cellular respiration in the light and in the dark 1. Graph your results. Either graph Asso or color/pH. Label the y-axis with Asso or pH value intervals that are appropriate to your data and label the x-axis with time in minutes. Plot the change versus time for both samples on the same graph. Use a ruler to draw a best fit line for the linear region of your light and dark datasets. Use a different color to plot your light and dark results, or use different kinds of lines (e.g. solid or dashed).

Answer 1

light dark + Abs550 B 10 50 203040 Time

Time in murs $5.54 light initial (4) light filial (24) Dark initial (pl) - og DO +01 Slope light = 0.02 stoppe dark & 0/0026