Question

9. Transcribe the top strand of this DNA into RNA. Label the 3' and 5' ends. DNA: 3' TAC CCC GGG AAA TTT ACT 5' 5' ATG GGG CCC TTT AAA TGA 3' S'AnGGGG CCc nUM AAA UGA? 10. Now translate your mRNA into Protein. Label the C-term and N-term.

Answer 1

10.) The mRNA transcribed from the DNA is encoded in 5'-3' direction by RNA polymerase. Right after it is called primary transcript and it contains long stretch of introns which are basically junk part of mRNA. After post transcriptional modifications it goes to the cytoplasm where mRNA is translated into amino acids.

5'- AUG GGG CCC UUU AAA UGA -3' (mRNA sequence)

N- Met Gly   Pro Phe Lys Stop- C (Amino acid sequence)

N-terminus- Methionine- Glycine- Proline- Phenylalanine- Lysine- Stop codon- C terminus

These amino acid chains are joined together with peptide bonds which makes the primary structure of proteins. Further after folding of the primary structure of proteins, they makes the secondary, tertiary structure of different proteins.