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During Drosophila development, adult structures are formed from clusters of cells called imaginal discs, which are...
You decide to make a construct that places the DNA between the genes upstream to a...
You decide to make a construct that places the DNA between the
genes upstream to a reporter gene (GFP) in place of Gene 1. The
full-length construct has the correct expression pattern in
antennal discs. You then create several constructs that delete
sections of the upstream DNA and score them for expression. The
seven constructs are shown below; the black bars indicate the
region(s) deleted in each construct. The data table on the right
shows the expression results for each...
ASSIGNMENT 1: DROSOPHILA IMAGINAL DISC DEVELOPMENT 1) In Drosophila, the legs, wings and other adult structures...
ASSIGNMENT 1: DROSOPHILA IMAGINAL DISC DEVELOPMENT 1) In Drosophila, the legs, wings and other adult structures develop from imaginal discs. Briefly describe what an imaginal disc is and how it forms an adult structure. 2) Describe the antero-posterior patterning of the dorsal wing imaginal disc, including the major genes involved and their role in this patterning. 3) Describe the antero-posterior, dorso-ventral and proximo-distal patterning of the ventral leg imaginal disc, including the major genes involved and their role in patterning.
You are studying the regulatory DNA of a mouse gene expressed in developing heart, liver, and...
You are studying the regulatory DNA of a mouse gene expressed in developing heart, liver, and lung tissue. Your preliminary work has shown that heart and lung expression of this gene is controlled by a short fragment of DNA just upstream of the promoter. Based on this result, you decide to investigate this region further to understand its function. You decide to compare this sequence to the regulatory DNA of the same gene found in rats and humans. Using genome...
1. Gene B is usually expressed only in skin cells. To learn about the mechanism by...
1. Gene B is usually expressed only in skin cells. To learn about the mechanism by which expression of the gene B is regulated, you make clones that contain a GFP reporter and various parts of the upstream and downstream intergenic regions of genomic DNA that normally surround gene B (black lines) as shown in the figure below. The resulting clones were introduced into frog skin cells growing in the lab and levels of GFP expression was monitored by measuring...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the DNA immediately upstream of the transcription start site, but expression of the gene can also be affected by control elements. These can be thousands of base pairs upstream of the promoter, grouped in an enhancer. Because the distance and spacing of these control elements make them difficult to identify, scientists begin by deleting sections of DNA that contain possible control elements and measuring the...
You decide to make a construct that places the DNA between the
genes upstream to a reporter gene (GFP) in place of Gene 1. The
full-length construct has the correct expression pattern in
antennal discs. You then create several constructs that delete
sections of the upstream DNA and score them for expression. The
seven constructs are shown below; the black bars indicate the
region(s) deleted in each construct. The data table on the right
shows the expression results for each...
ASSIGNMENT 1: DROSOPHILA IMAGINAL DISC DEVELOPMENT 1) In Drosophila, the legs, wings and other adult structures develop from imaginal discs. Briefly describe what an imaginal disc is and how it forms an adult structure. 2) Describe the antero-posterior patterning of the dorsal wing imaginal disc, including the major genes involved and their role in this patterning. 3) Describe the antero-posterior, dorso-ventral and proximo-distal patterning of the ventral leg imaginal disc, including the major genes involved and their role in patterning.
You are studying the regulatory DNA of a mouse gene expressed in developing heart, liver, and lung tissue. Your preliminary work has shown that heart and lung expression of this gene is controlled by a short fragment of DNA just upstream of the promoter. Based on this result, you decide to investigate this region further to understand its function. You decide to compare this sequence to the regulatory DNA of the same gene found in rats and humans. Using genome...
1. Gene B is usually expressed only in skin cells. To learn about the mechanism by which expression of the gene B is regulated, you make clones that contain a GFP reporter and various parts of the upstream and downstream intergenic regions of genomic DNA that normally surround gene B (black lines) as shown in the figure below. The resulting clones were introduced into frog skin cells growing in the lab and levels of GFP expression was monitored by measuring...
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