Homework Answers
11)
2)Uncouplers are a wide range
of unrelated chemical compounds that decrease mitochondrial ATP
synthesis but stimutes electron transport .They work by making the
inner mitochondrial membrane leaky to protons wchich prevents the
build up oh H+ ions suficient for ATP synthesis
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1. You are studying an in vitro suspension of mitochondria that does not contain any NADH....
Question 1 The mitochondria from a patient suffering from muscle pain during physical exercises were analyzed...
Question 1
The mitochondria from a patient suffering from muscle pain
during physical exercises were analyzed to detect any defect in one
of the components of the respiratory chain. Isolated mitochondria
were incubated in an isotonic buffer, and changes in the oxygen
consumption were measured as various components were sequentially
added to the reaction.
1) The following graph (Fig. 1) shows the changes of oxygen
concentration in a solution containing the patient’s mitochondria
or mitochondria from a healthy donor (control)....
Question 1 (25 points). The mitochondria from a patient suffering from muscle pain during physical exercises...
Question 1 (25 points).
The mitochondria from a patient suffering from muscle pain
during physical exercises were analyzed to detect any defect in one
of the components of the respiratory chain. Isolated mitochondria
were incubated in an isotonic buffer, and changes in the oxygen
consumption were measured as various components were sequentially
added to the reaction.
Part 1
The following graph (Fig. 1) shows the changes of oxygen
concentration in a solution containing the patient’s mitochondria
or mitochondria from a...
- I believed the inhibited protein was ATP synthase, however the data on Figure 2 does...
- I believed the inhibited protein was ATP synthase, however the
data on Figure 2 does not support this. Please help!
The mitochondria from a patient suffering from muscle pain during physical exercises were analyzed to detect any defect in one of the components of the respiratory chain. Isolated mitochondria were incubated in an isotonic buffer, and changes in the oxygen consumption were measured as various components were sequentially added to the reaction. 1) The following graph (Fig. 1) shows...
Can I get some help with question 1. a and b please? Uncouplers of oxidative phosphorylation...
Can I get some help with question 1. a and b please?
Uncouplers of oxidative phosphorylation in mitochondria inhibit the coupling between the electron transport and phosphorylation reactions and thus inhibit ATP synthesis without affecting the respiratory chain and ATP synthase (H(+)-ATPase). Miscellaneous compounds are known to be uncouplers, but weakly acidic uncouplers are representative because they show very potent activities. The most potent uncouplers discovered so far are the hindered phenol SF 6847, and hydrophobic salicylanilide S-13, whic are...
Create graphs for Figures 1-4 (circled on pages 111 & 114) based on the data given in Tables ...
Create graphs for Figures 1-4 (circled on pages 111 & 114)
based on the data given in Tables 2 & 4.
Lab # 8 Cellular Respiration and Fermentation I. Goals and Objectives At the completion of this laboratory exercise, students will be able to: A Differentiate between the intermediates and products of fermentation versus acrobic cellular respiration in yeast. Relate rates of fermentation with sugar availability in yeast. Utilize a reduction-oxidation dye to determine the effect of varying yeast concentration...
just the prelab worksheet, no data yet Lab Six: Fermentation Learning Objectives: • Explain the biochemistry...
just the prelab worksheet, no data yet
Lab Six: Fermentation Learning Objectives: • Explain the biochemistry of fermentation, substrates and products, conditions, and purpose for cells • Describe alcoholic fermentation of yeast, naming reactants and products Perform a pre-designed experiment to measure the rate of yeast fermentation of glucose under two different conditions. Propose hypotheses and make predictions based on them. Design and perform a novel experiment to test additional substrates for yeast fermentation using findings of the pre-designed experiment....
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
Question 1
The mitochondria from a patient suffering from muscle pain
during physical exercises were analyzed to detect any defect in one
of the components of the respiratory chain. Isolated mitochondria
were incubated in an isotonic buffer, and changes in the oxygen
consumption were measured as various components were sequentially
added to the reaction.
1) The following graph (Fig. 1) shows the changes of oxygen
concentration in a solution containing the patient’s mitochondria
or mitochondria from a healthy donor (control)....
Question 1 (25 points).
The mitochondria from a patient suffering from muscle pain
during physical exercises were analyzed to detect any defect in one
of the components of the respiratory chain. Isolated mitochondria
were incubated in an isotonic buffer, and changes in the oxygen
consumption were measured as various components were sequentially
added to the reaction.
Part 1
The following graph (Fig. 1) shows the changes of oxygen
concentration in a solution containing the patient’s mitochondria
or mitochondria from a...
- I believed the inhibited protein was ATP synthase, however the
data on Figure 2 does not support this. Please help!
The mitochondria from a patient suffering from muscle pain during physical exercises were analyzed to detect any defect in one of the components of the respiratory chain. Isolated mitochondria were incubated in an isotonic buffer, and changes in the oxygen consumption were measured as various components were sequentially added to the reaction. 1) The following graph (Fig. 1) shows...
Can I get some help with question 1. a and b please?
Uncouplers of oxidative phosphorylation in mitochondria inhibit the coupling between the electron transport and phosphorylation reactions and thus inhibit ATP synthesis without affecting the respiratory chain and ATP synthase (H(+)-ATPase). Miscellaneous compounds are known to be uncouplers, but weakly acidic uncouplers are representative because they show very potent activities. The most potent uncouplers discovered so far are the hindered phenol SF 6847, and hydrophobic salicylanilide S-13, whic are...
Create graphs for Figures 1-4 (circled on pages 111 & 114)
based on the data given in Tables 2 & 4.
Lab # 8 Cellular Respiration and Fermentation I. Goals and Objectives At the completion of this laboratory exercise, students will be able to: A Differentiate between the intermediates and products of fermentation versus acrobic cellular respiration in yeast. Relate rates of fermentation with sugar availability in yeast. Utilize a reduction-oxidation dye to determine the effect of varying yeast concentration...
just the prelab worksheet, no data yet
Lab Six: Fermentation Learning Objectives: • Explain the biochemistry of fermentation, substrates and products, conditions, and purpose for cells • Describe alcoholic fermentation of yeast, naming reactants and products Perform a pre-designed experiment to measure the rate of yeast fermentation of glucose under two different conditions. Propose hypotheses and make predictions based on them. Design and perform a novel experiment to test additional substrates for yeast fermentation using findings of the pre-designed experiment....
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
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