Question

 HOW MANY of the following statements are true? 

 i) DNA polymerase moves along the template strand in the 5' to 3' direction. 

 ii) DNA polymerase requires a primer to initiate replication.

 iii) DNA polymerase forms a covalent bond between a phosphate group and a hydroxyl group.

 iv) Polymerization of DNA for both the lagging and the leading strands is in the 5' to 3' direction.

 v) Shortly after DNA replication has begun, you find two replication forks moving in opposite directions way for the origin of replication.

Answer 1

Statement 1 - DNA polymerase enzyme moves in the direction of 3' to5' end producing new strand in the direction of 5' to 3'end. So first statement is false.

statement 2 - A DNA polymerase enzyme requires primer to initiate replication, so it's a true statement.

Statement 3 - when a new nucleotide is added to adjacent nucleotide, a covalent bond -phosphodiester bond is formed between them. So it's true statment.

Statement 4 - this statement is also true. Polymerization always works in 5' to 3' end .

Statement 5 - true ( two replication forks are appeared just after the initiation of replication.)