You want to study the proximity of two residues in clamp region of a protein. Draw...
You want to study the proximity of two residues in clamp region of a protein. Draw a schematic of your protein and label two locations of your fluorophores. Explain how your fret fluorophores would determine if these two residues were close to one another
Homework Answers
if two proteins are in close
proximity then the chromophores attached with one protein which is
donor if become excited ,it will emit the signal at emission
wavelength and the emission frequency is the excitaction frequency
of acceptor chromophore and then after excitation the donor
chromophore will emit the signal at its emission wavelength but
which is different from donor chromophore emission wavelength.
Now if two proteins are distantly placed then the do or molecules will not be excited and then you will only get the emission at the emission wavelength of donor chromophore.
Thank you
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