Homework Answers
Answer-
Lac operon consists of two kinds of genes:
1) Structural genes- There are 3 structural genes- Lac Z which codes for beta- galactosidase enzyme; Lac Y gene which codes for lactose permease; Lac A which codes for transacetylase enzyme.
2) Control genes- Regulatory gene which lies very upstream to promoter and produces a repressor. Upstream to regulatory gene there is i promoter. There are promoter-operator region which are present just upstream to lac operator. Transcription begins at promoter, where RNA polymerase binds. E. coli contains a protein called CAP which is encoded by a gene called crp. It is a positive regulator of lac operon. CAP site is located upstream to lac promoter.
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Lac Promoter i promoter galactoside transacetylase gene E coll chromosome lac operon loc terminator regulatory gene...
The lac operon contains three genes: lacZ, lacY, and lacA for β‑galactosidase, galactoside permease, and thiogalactoside transacetylase, respectively.
The lac operon contains three genes: lacZ, lacY, and lacA for β‑galactosidase, galactoside permease, and thiogalactoside transacetylase, respectively. The operon also contains a promoter site and an operator site. The regulatory gene includes the I gene for the Lac repressor protein and a promoter for that gene.The lac operon undergoes negative regulation. In the normal condition, the Lac repressor protein is active. Allolactose, an isomer of lactose, is the signal molecule that binds to the Lac repressor. Determine which events...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
on strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of...
on strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. Tones where proteins bind promoter DNA genes and regulatory sequences 100 mm n galactosidase proteins B-galacto de branscetylave perman Lac A promoter Lacz Lac! Operator Lacy
Match the protein listed below with the gene that encodes it or with the DNA sequence...
Match the protein listed below with the gene that encodes it or with the DNA sequence within the lac operon where it binds: lac repressor (encoded by...) [Choose] lac repressor (where it binds...) [Choose ] B-galactosidase [Choose] Lactose permease [Choose] Galactoside transacetylase [Choose] CAP [Choose] lacz lacy lacl Operator CAP site lacA
the answer I gave was wrong A mutant E. coli strain is found that synthesizes B-galactosidase...
the answer I gave was wrong
A mutant E. coli strain is found that synthesizes B-galactosidase whether or not lactose (or allolactose) is present. What mutations can independently lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences lac 1 operator promoter -35 lac z lac Y lac A JOSSA expression Lac repressor B-galactosidase B-galactoside- transacetylase proteins permease Lac Z Lac A promoter operator laci Lac Y
A) An Operon is:? 1- A gene that affect another gene transcription. 2. A gene coding...
A) An Operon is:? 1- A gene that affect another gene transcription. 2. A gene coding for a metabolic pathway. 3. A protein that blockgene expression. 4. A set of genes under the control of one promoter. B )- In bacteria the Lac Operon genes code for proteins that break down.? 1- Arabinose 2- Lactose 3- Galactose 4- Glucose C )mRNA processing involves removal of .......? 1- Introns 2- The poly-A tail 3- The 5' cap 4- Exons D )...
Please solve all of them Lac Mutants 1-Copy 2 of 10 CAP laclCAP gene lacO lacP...
Please solve all of them
Lac Mutants 1-Copy 2 of 10 CAP laclCAP gene lacO lacP acolacZacY Carbon source in theLac operon p-galactosidaseLactose permease binding site me levels in levels in the cell polycistronic mRNA enzy levels in the cell Undetectable Low but detectable Low but deteteable Low but detectable medium the cell Undetectable membrane Glucose only Glucose and lactose Lactose on Another carbon source Undetectable Undetectable Undetectable Undetectable The table shows the results of experiments measuring expression of the...
The lac operon consists of a promoter that initiates transcription of the gene i, which encodes...
The lac operon consists of a promoter that initiates
transcription of the gene i, which encodes a repressor protein. A
seperate promoter, p, within the operon initiates transcription of
z, which encodes B-galactosidase, and y, which encodes permease.
The operator, o, where the repressor protein binds to inhibit
transcription, is positioned between mutations, whereas superscript
c denotes a constitutively active mutant. The table below sea fives
the activity of B-galatosidase and permease for haploid and diploid
mutant strains. Match the...
Background: Several genetic regions are needed for lactose metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene), lacY(permease gene), lacA(transacetylase...
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
on strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. Tones where proteins bind promoter DNA genes and regulatory sequences 100 mm n galactosidase proteins B-galacto de branscetylave perman Lac A promoter Lacz Lac! Operator Lacy
Match the protein listed below with the gene that encodes it or with the DNA sequence within the lac operon where it binds: lac repressor (encoded by...) [Choose] lac repressor (where it binds...) [Choose ] B-galactosidase [Choose] Lactose permease [Choose] Galactoside transacetylase [Choose] CAP [Choose] lacz lacy lacl Operator CAP site lacA
the answer I gave was wrong
A mutant E. coli strain is found that synthesizes B-galactosidase whether or not lactose (or allolactose) is present. What mutations can independently lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences lac 1 operator promoter -35 lac z lac Y lac A JOSSA expression Lac repressor B-galactosidase B-galactoside- transacetylase proteins permease Lac Z Lac A promoter operator laci Lac Y
Please solve all of them
Lac Mutants 1-Copy 2 of 10 CAP laclCAP gene lacO lacP acolacZacY Carbon source in theLac operon p-galactosidaseLactose permease binding site me levels in levels in the cell polycistronic mRNA enzy levels in the cell Undetectable Low but detectable Low but deteteable Low but detectable medium the cell Undetectable membrane Glucose only Glucose and lactose Lactose on Another carbon source Undetectable Undetectable Undetectable Undetectable The table shows the results of experiments measuring expression of the...
The lac operon consists of a promoter that initiates
transcription of the gene i, which encodes a repressor protein. A
seperate promoter, p, within the operon initiates transcription of
z, which encodes B-galactosidase, and y, which encodes permease.
The operator, o, where the repressor protein binds to inhibit
transcription, is positioned between mutations, whereas superscript
c denotes a constitutively active mutant. The table below sea fives
the activity of B-galatosidase and permease for haploid and diploid
mutant strains. Match the...
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
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