Question

Can you answer these questions pleasee: If we performed electrophoretic separation (AGE) of human genomic DNA (buccal swab extraction), and we didn’t perform any restriction digestion, answer the following:

Yellow arrows indicate loaded samples (all genomic DNA – uncut) Blue arrow indicates DNA ladder (AKKA marker) A green arrow indicates loading of a cut plasmid (what would happen if we performed restriction digestion of the plasmid with an enzyme that has only one restriction enzyme site) Yellow rectangle shows you what is "lane”, where your samples are separated according to length... so in total, you have 13 lanes (1, 2, ... 11, M and uncut plasmid) Black arrow points to the "wells”, where you load the samples Dashed orange lines are here just to make easier for you to understand the orientation of separated bands. IIIIIIIIIIT -2.000 -2,000 III 888888888 ITU 2u1 LD-47U DNA sample

a)In your opinion, which of the loaded samples has the highest and lowest concentration of DNA, respectively?

b)Indicate the lanes in which DNA is degraded and explain your answer.
c)Take a look into the lane “cut Plasmid”. Explain the appearance of two bands. What kind of a technique was performed on this sample that hasn’t been performed on other samples?

Thank you in advance. I really need your help and I would appreciate it very very much!!

Answer 1

a.Sample 1 and 7 has the highest concentration while lane 6 has the lowest concentration of DNA.

b.In lane 9 and 11 the DNA has degraded.It may be due to nuclease activity

c.Two bands are seen due to restriction digestion that gives rise to one linear DNA and one circular due to incomplete digestion