Question

Can you pleasee answer these questions: If we performed electrophoretic separation (AGE) of human genomic DNA (buccal swab extraction), and we didn’t perform any restriction digestion, answer the following:
1. How many bands do you expect to see in each lane where genomic DNA is loaded?
2. Why do you see bands above the molecular marker?
3. How many wells do you see on the gel? How many of them are used in the experiment (i.e. have loaded samples)?

Thank you in advance, please help me, I am eternally grateful for your help!!!

Answer 1

1. There will be only one thick band of genomic DNA present upper in the lane if it is not treated with restriction endonuclease enzymes . Lowe in the well you may observe some smearing due to RNA contamination.

2. As genomic DNA is so large , so it will be unable to move through the gel so it will be present near the well in the gel .

3. For this please provide the gel image . Wells having band will be experimental Wells.