Ans :
The Mitochondria are sometimes called the “power house” of the cell because of their role in energy production.Detecting mitochondrial metabolic changes is vital to the understanding of mitochondrial disorders and responses to pharmacological agents. Mitochondrial metabolic changes are so difficult to distinct and hence may be hindered by other cytosolic metabolites obtained using whole organism or whole tissue extractions.By doing Homogenate fraction they limit contamination of cytosolic metabolites in the enriched mitochondrial fraction. Also provide a more accurate quantification of mitochondrial specific metabolites. Using this fraction we can obtain high quantity, quality and variety of metabolites by enriching mitochondria to reveal distinct metabolite shifts and changes.
Why would mitochondria be enriched in Homogenate fraction when no fractionation has taken place ? Specific...
1.
Why would the homogenate fraction have the highest succinate
dehydrogenase activity and thus the most mitochondria ( more than
the S1,P1,S2 and P2 fraction).
SDH activity Initial Velocity (Abs./s) P1 Fractions Figure 2. The SDH of each fraction measured in velocity (Abs./sec). The H fraction had the highest initial velocity of 0.057 Abs./s while the P2 fraction had the lowest initial velocity of 0.0033 Abs./s.
5. When all of the sample to be collected has left the
column, will the drop of solution coming off the tip of the column
be acidic, basic or neutral?
6. How many H+ ions would be displaced from the ion
exchange column by 1 Cu2+ ion?
7. Binding sites on the ion exchange resin are saturated
with which ion before the sample solution is added?
8. Ions are displaced from the resin binding sites by
any ion that has...
1. Analyze the major challanges USB faced in the last 5 years, in your opinion, what were the crucial factors in the banks downturn? 2. what are the main triggers to change the banks approach to communication and what is different today regarding the dealings and relationship to its share-and stakeholders? 3. How would you evaluate the constant replacement of the banks chairman and CEO? 4. in view of the future strategy of USB, what are your suggestions in order...
1. Analyze the major challanges USB faced in the last 5 years, in your opinion, what were the crucial factors in the banks downturn? 2. what are the main triggers to change the banks approach to communication and what is different today regarding the dealings and relationship to its share-and stakeholders? 3. How would you evaluate the constant replacement of the banks chairman and CEO? 4. in view of the future strategy of USB, what are your suggestions in order...
Based on the document below,
1. Describe the hypothesis Chaudhuri et al ids attempting to
evaluate; in other words, what is the goal of this paper? Why is he
writing it?
2. Does the data presented in the paper support the hypothesis
stated in the introduction? Explain.
3.According to Chaudhuri, what is the potential role of thew
alkaline phosphatase in the cleanup of industrial waste.
CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...
explaim the mechanisms amd toxological effects if type 1
diabetes in this article
Exposure to arsenic in drinking water is associated with increased prevalence of diabetes. We previously reported an association of diabetes and urinary concentration of dimethylarsinite (DMAS"), a toxic product of arsenic methylation by arsenic (+ 3 oxidation state) methyltransferase (AS3MT). Here we examine associations between AS3MT polymorphism, arsenic metabolism and diabetes. Fasting blood glucose, oral glucose tolerance and self-reported diagnoses were used to identify diabetic individuals. Inorganic...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...
I need Summary of this Paper i dont need long summary i need
What methodology they used , what is the purpose of this paper and
some conclusions and contributes of this paper. I need this for my
Finishing Project so i need this ASAP please ( IN 1-2-3 HOURS
PLEASE !!!)
SPECIAL ARTICLES tole of Monetary Policy C Rangarajan What should be the objectives of monetary policy? Does the objective of price stability conflict with the goal of achieving...