In what layer, organic or inorganic, is the DNA during a the phenol:chloroform extraction?
In what layer, organic or inorganic, is the DNA during a the phenol:chloroform extraction?
Homework Answers
When we add phenol chloroform to the isolation mixture during DNA extraction, than two layers are formed, upper aqueous layer and lower organic layer.
The lower organic layer contains all the biomolecules but the upper aqueous layer contains DNA which is solubilized into it.
The aqueous layer is than transferred to a new tube amd ethanol is added to precipitate out DNA.
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In what layer, organic or inorganic, is the DNA during a the
phenol:chloroform extraction?
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Complete the reactions equations for the chemistry that took
place when the organic layer was extracted. Cross out analyte that
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help please An extraction was performed using mixture of the compounds below. First the organic mixture...
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Prelab
1. Explain in a few sentences how extraction can be used to separate an organic compound from
sodium chloride.
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3. In general, how...
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extraction, instead of ether, what would the top layer be in the
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experiment.
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Complete the reactions equations for the chemistry that took
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opts) Complete the reaction equations for the chemistry that took place when the organic layer was extracted with aqueous acid (reaction with H2O*). Cross out the analyte that is no longer present in the organic layer during the extraction with aqueous acid (i.e. the analyte that was extracted by...
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help please
An extraction was performed using mixture of the compounds below. First the organic mixture was dissolved in dichloromethane, treated with acid, and separated. The organic layer was then treated with base and separated. Sort the compounds based which layer it was in at the end of the extraction.
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