Homework Answers
The incubation temperature of both the petriplates is different. The 1st plate shows growth of ura3- strain, diploid X-Y, X strain and Y strain at a temperature of 23 degree. However in the 2nd plate where the incubation temeperature is 32 degree, only ura3- and diploid X-Y strains are able to grow. The mutants X and Y strains do not show growth at 32 degree. This essentially means that X and Y are mutants where genes are mutated which helps them to grow individually like ura3- strain. And the growth of diploid X-Y at 32 degree means that the mutated genes complement eachother so that when they are present together in a cell, a fully functional gene is formed and the diploid is able to grow at 32 degree.
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Culture of Yeast that requires uracil for growth (ura3-) Two mutants that are ura3- have been...
D Question 2 13 pts A culture of yeast that requires uracil for growth (ura3 )...
D Question 2 13 pts A culture of yeast that requires uracil for growth (ura3 ) was treated with a mutagen, and two mutant colonies, X and Y, were isolated. Mating type a cells of mutant X were mated with mating type a cells of mutant Y to form diploid cells. The parental (ura3), X, Y, and diploid cells were all streaked onto agar plates containing uracil and incubated at 23 "C or 32 °C. Cell growth was monitored by...
CDNA Library Screen Growth at 23 °C on Growth at 32 °C on media lacking uracil...
CDNA Library Screen Growth at 23 °C on Growth at 32 °C on media lacking uracil media lacking uracil • Prepared a cDNA library from the wild-type URA3+ cells, which is used to transformed into X cells Each spot represents a single clone growing What are the MOLECULAR differences between clones growing on the two plate? How can these results be used to identify the plasmid containing the wild-type URA3+ gene? Based on these results, how can the identity of...
KHomework II Practice Problem 115 Review Three haploid yeast petite mutants were isolated one from each of the thr...
KHomework II Practice Problem 115 Review Three haploid yeast petite mutants were isolated one from each of the three classes of petite mutations-suppressive petites, neutral petites, and segregational petites. You perform the crosses shown in this table. Progeny (haploid spores) Cross 50% petite: 50% wild petite #1 x wild type type petite #2 x wild type 100% petite P petite #3 x wild type 100% wild type Se on WoL mini ferm Whic coul mutant ( will segregate in a...
2. A homolog of your favorite gene, YFG1, has been identified in S. cerevisiae. You were...
2. A homolog of your favorite gene, YFG1, has been identified in S. cerevisiae. You were tasked with: i. generating a null allele of YFG1 in a diploid strain with the genotype: his3/his3, TRP1/trp1, ura3/URA3, YFG/YFG using a one-step gene replacement. [ So after the gene replacement genotype will be his3/his3, TRP1/trp1, ura3/URA3, yfg:marker/YFG] i. Sporulating the knockout strain generated in (i) iii. dissecting the resulting tetrads (asci) iv. testing the resulting spores on a variety of growth media The...
e) Can you identify any wildtype (nonmutant) colonies in the figures? Explain your answer. f) Can...
e) Can you identify any wildtype (nonmutant) colonies in the figures? Explain your answer. f) Can you identify any standard (non-conditional) mutants in the figures? Explain your answer. g) Can you identify any conditional mutants in the figures? Explain your answer. 23°C mutant colony in which cells proliferate at the cooler, permissive temperature but fail to proliferate at the warmer, nonpermissive temperature 23°C colonies replicated onto two identical plates and incubated at two different temperatures mutagenized cells plated out in...
A haploid strain of yeast is treated with a mutagen called EMS. The different steps in...
A haploid strain of yeast is treated with a mutagen called EMS. The different steps in the treatment and the analysis are the following: Step 1: Two tubes, labeled A and B contain 0.2 ml each of same yeast liquid culture. The concentration of yeast cells in the liquid culture was determined by a measure of the turbidity of the culture. This concentration is 4.4 x 109 cells/ml. Step 2: In each tube (A and B) 1.5 ml of phosphate...
The adaptation phase for the E. coli culture lasted A culture of E. coli was grown...
The adaptation phase for the E. coli culture lasted
A culture of E. coli was grown on suitable culture media; aliquots of the culture were serially diluted and spread plated aseptically on agar plate media at the times indicated in the table below. Upon overnight incubation at 37°C, colonies appeared and viable counts were colleted and displayed below. Hours 0 Viable Counts (CFU/mL) 2 .00 x 107 2.10 x 107 2.30 x 107 3.25 x 107 6.60 x 107 1.40...
In order to count cells, the culture must be duted The technique o serialul precise and...
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing enough growth factors. Which of the following graphs corresponds to these cells when their DNA content is analyzed 24 hours after they are induced to express high levels of a mutant form of Rb protein that cannot be phosphorylated? (Note: this cell line has its cell cycle around 22 hours) Control cells #1 #2 #3 #4 #5 #6 Cell number JAN ul. 2 2...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
D Question 2 13 pts A culture of yeast that requires uracil for growth (ura3 ) was treated with a mutagen, and two mutant colonies, X and Y, were isolated. Mating type a cells of mutant X were mated with mating type a cells of mutant Y to form diploid cells. The parental (ura3), X, Y, and diploid cells were all streaked onto agar plates containing uracil and incubated at 23 "C or 32 °C. Cell growth was monitored by...
CDNA Library Screen Growth at 23 °C on Growth at 32 °C on media lacking uracil media lacking uracil • Prepared a cDNA library from the wild-type URA3+ cells, which is used to transformed into X cells Each spot represents a single clone growing What are the MOLECULAR differences between clones growing on the two plate? How can these results be used to identify the plasmid containing the wild-type URA3+ gene? Based on these results, how can the identity of...
KHomework II Practice Problem 115 Review Three haploid yeast petite mutants were isolated one from each of the three classes of petite mutations-suppressive petites, neutral petites, and segregational petites. You perform the crosses shown in this table. Progeny (haploid spores) Cross 50% petite: 50% wild petite #1 x wild type type petite #2 x wild type 100% petite P petite #3 x wild type 100% wild type Se on WoL mini ferm Whic coul mutant ( will segregate in a...
2. A homolog of your favorite gene, YFG1, has been identified in S. cerevisiae. You were tasked with: i. generating a null allele of YFG1 in a diploid strain with the genotype: his3/his3, TRP1/trp1, ura3/URA3, YFG/YFG using a one-step gene replacement. [ So after the gene replacement genotype will be his3/his3, TRP1/trp1, ura3/URA3, yfg:marker/YFG] i. Sporulating the knockout strain generated in (i) iii. dissecting the resulting tetrads (asci) iv. testing the resulting spores on a variety of growth media The...
e) Can you identify any wildtype (nonmutant) colonies in the figures? Explain your answer. f) Can you identify any standard (non-conditional) mutants in the figures? Explain your answer. g) Can you identify any conditional mutants in the figures? Explain your answer. 23°C mutant colony in which cells proliferate at the cooler, permissive temperature but fail to proliferate at the warmer, nonpermissive temperature 23°C colonies replicated onto two identical plates and incubated at two different temperatures mutagenized cells plated out in...
The adaptation phase for the E. coli culture lasted
A culture of E. coli was grown on suitable culture media; aliquots of the culture were serially diluted and spread plated aseptically on agar plate media at the times indicated in the table below. Upon overnight incubation at 37°C, colonies appeared and viable counts were colleted and displayed below. Hours 0 Viable Counts (CFU/mL) 2 .00 x 107 2.10 x 107 2.30 x 107 3.25 x 107 6.60 x 107 1.40...
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing enough growth factors. Which of the following graphs corresponds to these cells when their DNA content is analyzed 24 hours after they are induced to express high levels of a mutant form of Rb protein that cannot be phosphorylated? (Note: this cell line has its cell cycle around 22 hours) Control cells #1 #2 #3 #4 #5 #6 Cell number JAN ul. 2 2...
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