Question

8. Bacterial and eukaryotic mRNA polymerases two ions that coordinate two residues in the protein active site. (2 pts) 9. Class I and Il aminoacyltRNA synthetases both use a intermediate Class synthetases attach this intermediate to the 2' OH of a(n) _ synthetases attach this intermediate to the the tRNA 3' end, while Class 3' OH on the 3' end of a tRNA. (4 pts) 10. Group Il intron mechanism, a the - of a(n) residue within the intron serves as a intermediate is formed that contains a _phosphodiester bond (6 pts) bond cleavage result in charged species, while bond cleavage results in the generation of radicals. In biological systems, bond cleavages at carbon are most commonly -- (3pts) 12. The double bonds in unsaturated fatty acids are commonly in a _configuration, which results in the chain being _. (2 pts)

Answer 1

8.Regulatory, structural.

9.trna , class I aminoacyltrna, class II aminoacyltrna.

11.Heterolytic, homolytic ,heterolytic cleavage

Heterolytic bond cleavage : Bond breaking in which the bonding electron pair is split unevenly between the products. Heterolytic cleavage often produces at least one ion.

Homolytic bond cleavage : Bond breaking in which the bonding electron pair is split evenly between the products. Homolytic cleavage often produces radicals.

12.Cis, trans configuration

In nature, cis configuration is the most common one for unsaturated fatty acids.when the chain enters and exits on opposite sides of the plan, double bond is defined in trans configuration.