A protein in which its binding to the operator inhibits the transcription of one or more genes
Repressor proteins can be DNA–or RNA-binding
DNA-binding repressors - block the binding of RNA
polymerase to the promoter. As a result, the gene is prevented from
being transcribed into mRNA
RNA-binding repressors - bind to mRNA, preventing
protein translation
Examples of repressor proteins are lac repressor that inhibits the
expression of lac operon in E. coli. Another is MetJ, a methionine
repressor of met operon.
Repressor proteins are influenced by the presence of other
molecules, such as corepressors
and inducers. Corepressors are molecules
that bind and activate repressors. Inducers, on the other hand,
bind and regulate repressors by inducing the latter to undergo
conformational change thereby decreasing binding affinity to the
operator.
Select all characteristics of repressor proteins. Check Al That Apply Able to bind is acting elements...
on Select all characteristics of proteins that are positive regulators of transcription Check All That Apply Positive regulators of transcription never need to associate with any other molecule to bind DNA. O Positive regulators help recruit ribosomes. O Positive regulatory proteins can affect only one operon. O Positive regulators often bind effectors before binding with DNA. O Activators regulate some degradative pathways. O Positive regulators help recruit RNA polymerase. Prey e to search
1. trans-acting factors are able to regulate target genes from any chromosome, whereas cis-acting elements can only regulate genes located in the same chromosome. a. True only in operons. In Eukaryotic systems, trans-acting factors only regulate genes in the same chromosome. b. True only in Eukaryotes. Prokaryotes don’t have cis-acting elements. c. True for any organism. d. False. The statement is erroneous 2. Unlike activators, repressors never affect chromatin structure. Repressors inhibit transcription only by binding to the binding sites...
A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the structural genes with the reporter gene green fluorescent protein (GFP). The repressor allele cannot bind lactose, and the OC allele cannot be bound by the repressor. Given the following genotypes and growth media that does not contain glucose, indicate in the table below whether you expect to see b-galactosidase activity and glowing green colonies of E. coli by selecting Yes or No. growth media...