C5 is a pilus-specific phage and E. coli has pili so why is there no clearing...
C5 is a pilus-specific phage and E. coli has pili so why is there no clearing zone when C5 is spotted on E. coli?
Homework Answers
In a plaque assay, phage particles are introduced in a medium containing bacterial cells. Phage is able to infect bacterial cells by binding to specific receptors.
A clearing zone is observed in the plate if bacterial cells get infected by phage due to antigenic compatibility. Phage particles kill bacterial cells and A zone of clearance is formed.
However, if C5 particle lacks antigenic determinant, then it won't be able to infect and kill bacterial cells, even if the phage is pilus-specific. In such a scenario, bacterial cells would continue to grow and there would be no clearing zone.
Hence, C5 won't be able to infect and lyse bacterial cells if it lacks antigenic determinant . If there is no lysis, zone of clearing cannot be observed.
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C5 is a pilus-specific phage and E. coli has pili so why is
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