A generalized transducing phage is used to transfer genes from a thr(+)bio(+)thi(+) donor strain of E. coli to a thr(-)bio(-)thi(-) recipient strain. When transductants selected on a medium containing thiamine and threonine are tested for growth on other media, many more colonies are found on media supplemented only with threonine than on media supplemented only with thiamine. No transductants survive on minimal media.
What is the correct order for these three genes?
*I have seen multiple different answers for this, either bio-thi-thr or thr-bio-tri so if you could explain which one it is and why that would be great!*
Which two genes are most closely linked?
A generalized transducing phage is used to transfer genes from a thr(+)bio(+)thi(+) donor strain of E....
Note whether in the following strains of E. coli, B-galactosidase will be: (A) Abundant in both the presence and absence of lactose (B) Abundant only in the absence of lactose (C) Abundant only in the presence of lactose (D) Abundant only in the presence of sucrose (D) Absent in both the presence and absence of lactose Can you answer and explain these please? What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
genetic question ASAP!!! (5) (10 0 points) In re ser pro* his ret cells are ser pro his (i.e. they will only grow if serine, proline and histidine are included in ne or h and the recipient cells are ser pro the media a generalized transduction system using P1 phage, the E. coli donor cells a transductants on re then tested on plates containing just minimal medihedonor allele ser is initially selected after transduction (by growing potential tras edia plus...
3. (7) Suppose that you have selected E. coli that can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis - cysX . You have two strains of bacteria: Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s F - cysX+ gal - his - lac - pur - trp - ton R A) What supplements would you need to add to...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...