Question

Order the steps to generate a transgenic mouse from when male and female mice are first mated to breeding heterozygous offspring together to generate a homozygote.

mate male and female mice

breed heterozygous offspring together to generate a homozygote

Answer Bank

Harvest Fertilized egg from mate females

Injected the transgene into pronucleus

Id integrants by DNA Analysis

Implant the embryos into a surrogate female

Answer 1

Transgenic techniques describes the process of introducing foreign deoxyribonucleic acid (DNA) into a host organism's Genome Transgenic techniques is a technique in which an organism (typically a mouse) that is engineered to carry a foreign gene, or transgene of choice as part of its own genetic material for Disease models and human welfare. Transgenics are genetically modified organisms with DNA from another source inserted into their genome. A large number of transgenic animals have been created such as Mice, Cows, Pigs, Sheep, Goats, Fish, and Frogs.

Transgenesis is the process of introducing an exogenous gene- called a transgene - into a living organism so that the organism will exhibit a new property and transmit that property to its offspring. Transgene: It is a gene or genetic material that has been transferred naturally or by any of a number of genetic engineering techniques from one organism to another. Transgenic Plants or animals : The plants or animals which expresses the characters coded by the transgene are called Transgenic plants or transgenic animals.

Mm Mm Heterozygous Heterozygous Knockout Wildtype mm Mm MM Homozygous Heterozygous 50% Wildtype 25% 25%

Select for cells expressing transgene Embryonic Stem (ES) cells ES CELL INJECTION Inner cell mass Blastocyst tools Implant in uterus Transgene PRO-NUCLEAR MICROINJECTION Male pro-nucleus Implant in uterus Fertilized egg Pseudo pregnant female Screen off spring for transgene Mate heterozygous offspring to each other to generate homozygous mice