Question

Order the steps to generate a transgenic mouse from when male and female mice are first mated to breeding heterozygous offspring together to generate a homozygote. mate male and female mice breed heterozygous offspring together to generate a homozygote Answer Bank harvest fertilized eggs from mated females implant the embryos into a surrogate female inject the transgene into a pronucleus identify integrants by DNA analysis

Answer 1

Their can be are two correct answers:

Steps are:.

A.

1.Identify intergrant by DNA analysis

2.Harvest fertilized eggs from mated females.

3. Inject the transgene into a pronucleus.

4. Implant the embryo into surrogate female.

B.

1. Harvest fertilized eggs from mated females

2. Identify integrants by DNA analysis

3. Inject the transgene into a pronucleus.

4. Implant the embryo into surrogate females.

(Both A and B are correct, it just the representation of answer which is different. Every reference book tell same thing with some variation. Select best answer as per your need).

Keep Chegging.