Question

help asap! easy! bio!

If you wanted to make 60 ml of a 1% agarose gol in TBE, how many grams of agarose do you need? What is the TBE used for in the gol and in the gol box? T TTT Paragraph : Arial 3(12pt) .E. T. %DO Q E T' T. - -

Answer 1

1. To make 100 ml of 1% agarose gel, 1 gram of agarose has to be mixed with 100 ml of TBE buffer.

100 ml of TBE needs 1 gram of agarose

1 ml of TBE needs 1/100 gram of agarose

60 ml TBE needs 1/100×60 = 0.6 gram of agarose.

So, to make 60 ml of 1% agarose gel in TBE, 0.6 gram of agarose has to be mixed with 60 ml of TBE buffer.

* Point to be noted that sometimes the amount of agarose to be added may vary according to the grade of that agarose. To get clear idea about that the information printed on the agarose container by the company should be followed.

2. Role of TBE and reason of using TBE :

• When TBE buffer is mixed with agarose, it helps in forming the gel by polymerization of the agarose and form a matrix which is stable at maintaining neutral pH
• Additionally when the TBE buffer is added to the box or the electrophoresis chamber, the weak acid and base in the buffer keep the pH of electrophoresis chamber in a desired range i.e. nearly neutral
• By maintaining neutral pH, TBE controls the net charge of molecules which basically helps in proper migration and separation of the molecules
• Moreover TBE buffer helps in an electric conductor in the eletrophoretic chamber.

**** IF YOU LIKE THE ANSWER PLEASE HIT THE THUMBS-UP. THANK YOU :-)