Question

denatures (unfolds) proteins Question 14 2 pts DNA barcoding regions were carefully selected. Which of the following is NOT a valid reason for using a DNA sequence for barcoding? The sequence is similar enough within a species to say those species are the same The sequence is divergent enough between species to tell them apart3 The flanking regions are similar enough so that universal primers can bind to and amplity almost any species within a kingdom The sequence is rich in As and Ts which base pair more strongly with the primers 1 pts Question 15 Gel #1 BRODA

Answer 1

The flanking regions are similar enough so that universal primer can bind to and amplify almost any species within a kingdom...

Because Beside the risk of contamination of the DNA sample by PCR inhibitors, primer bias is one of the major sources of errors in DNA barcoding. The isolation of an efficient DNA marker and the design of primers is a complex process and considerable effort has been made to develop primers for DNA barcoding in different taxonomic groups.However, primers will often bind preferentially to some sequences, leading to differential primer efficiency and specificity and unrepresentative communities’ assessment and richness inflation. Thus, the composition of the sample's communities sequences is mainly altered at the PCR step. ...