Question

4. Draw a phylogenetic tree of vertebrate MCSF proteins. Include in this tree only the following (2 points) mouse MCSF, human MCSF, zebrafish MCSFI, zebrafish MCSF2, salmon MCSF1, salmon MCSF2 and predict where the frog and the chicken MCSFs would branch. 5A. Are the different fish MCSF gene sequences more closely related to each other or to the mammalian MCSF gene sequences? 5B. According to the authors, what does this suggest about the functional roles of the fish MCSF molecule? SC. Explain why you agree or disagree with the authors in this regard. (3 points)

Answer 1

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The accession number of Cen: bank are Zebrafish (Danio retio) dr Mesti AM901598 is for goldfish, EX 88 1752 killifish, EB035272; Stickle hack, AM 949 839 salmon (Salmo Salar), dra98450 human (Homo sapiens Po9603) mouse! SA Answers The different fish mest qene sequence more closely related to - seach other. - Two Fish MOSF gene exist in Fish- After analysis of genome database and EST. I'mncsf molecale is zebrafish and alliften, Afontic hall but Ara two clades of an unrooles phylogentic free were formed anong the distinct and these are seperated from others cs and more thaner ' MESE cladd.

50 Ans 3 f The MCSF gene authors fish. had study on of with the help of these we can trace then d o immune response related study, major disase. 2) It was also detectable in outside of the immune system like brain of muscle 2) In head kidney the MESF 2 gene dompnate cohere as mest i gene 18 spleen, shows higher level in bony CS Scanned with Camscannesh