1) Using the map above, draw a picture of an appropriate targeting vector and draw a...

Question

Question

Review of Concepts: Gene Identification and Gene Knockout You want to study the physiological role of a gene you just cloned

1) Using the map above, draw a picture of an appropriate targeting vector and draw a map of the gene after the neomycin resistance gene (neo) has been inserted.

science biology

Add a comment Improve this question Transcribed image text

Answer 1

Answer #1

Gene. 2 Probe 2 Targeting Vector vector loxp Neo loxp Target Gene. Probe 10x P Neo loxp

Add a comment

Answer 2

Similar Homework Help Questions

2) You think that the neomycin resistance gene (neo) is causing an artificial result, so you...

2) You think that the neomycin resistance gene (neo) is causing an artificial result, so you also make a “knock-out” mutant using the LoxP-Cre system to splice-out the neo gene. Briefly, describe how you would accomplish this task. Draw a picture of the targeting vectors and a map of the gene before and after the neo gene has been removed Review of Concepts: Gene Identification and Gene Knockout You want to study the physiological role of a gene you just...
May you check if this answer is right and add any missing information to this answer...

May you check if this answer is right and add any missing information to this answer (for example the origin of replication, etc.) 1) Using the map above, draw a picture of an appropriate targeting vector and draw a map of the gene after neo has been inserted. Review of Concepts: Gene Identification and Gene Knockout You want to study the physiological role of a gene you just cloned by making a "Knock-out" mutant mouse. Your gene has 5 exons...
6a) You want to use homologous recombination to generate a mouse that does not express a functional XPC gene (‘Knock-out...

6a) You want to use homologous recombination to generate a mouse that does not express a functional XPC gene (‘Knock-out’ or KO). To do this, you want to delete exon 10 and replace it with a gene that confers resistance to the drug Neomycin (NEO. The usual second selection with tk we discussed in class is not shown here). Using the diagram as a guide: illustrate on the diagram where you expect crossing over to occur and (below the diagram)...

2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...

2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...

1) Using the map above, draw a picture of an appropriate targeting vector and draw a...

Homework Answers

Add Answer to:
1) Using the map above, draw a picture of an appropriate targeting vector and draw a...

Post as a guest

Earn Coins

2) You think that the neomycin resistance gene (neo) is causing an artificial result, so you...

May you check if this answer is right and add any missing information to this answer...

6a) You want to use homologous recombination to generate a mouse that does not express a functional XPC gene (‘Knock-out...

2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...

1) Using the map above, draw a picture of an appropriate targeting vector and draw a...

Homework Answers

Add Answer to: 1) Using the map above, draw a picture of an appropriate targeting vector and draw a...

Post as a guest

Earn Coins

2) You think that the neomycin resistance gene (neo) is causing an artificial result, so you...

May you check if this answer is right and add any missing information to this answer...

6a) You want to use homologous recombination to generate a mouse that does not express a functional XPC gene (‘Knock-out...

2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...

Add Answer to:
1) Using the map above, draw a picture of an appropriate targeting vector and draw a...