Question

(NOTE: This question will require information from Unit 6 in order to answer it). Problem 4.2.3 (tags: #SNARES, #vesicle transport) Consider the v-SNAREs that directly transport vesicles from the trans Golgi network (TGN) to the plasma membrane. They, like all other v-SNAREs, are membrane proteins that are integrated into the membrane of the ER during their biosynthesis, and are then transported by vesicles to their destination. Thus, transport vesicles budding from the ER contain at least two kinds of V-SNARES - those that target the vesicles to the cis Golgi and those that are in transit to the TGN to be packaged into different transport vesicles (as cargo) destined for the plasma membrane. a) Why do you suppose this might be a problem for the cell? b) How do you suppose the cell might solve this problem?

Answer 1

Mechanism of vesicle docking proteins involved 1SNARE protein : Soluble NSE Attachment Receplor protein : It has a entities 1, SNAP (soluble NSF attachment protein) SNARE " NSF protein I protein NSE - N- Ethylmaleimide Sensitive fusion protein Involved in Association and dissociation of SNARE Proteins SNARE protein W-SNARE t-SNARE present in vesicle present in target membrane membrane ER- Gogli interface --> protein traveport between the endoplasmic reticulum (ER) and Golgi is a highly dynamic росел ER will transfer protein to cis - Golgi by forming vesicle in ER which contains proteins (cop-II protein)

- If any protein got escaped to cis-golgi , then Cia-golg: will form a vesicle which contains cop-I protein, and that escaped protein will be sent back to ER slant ou proteins play major rolen 0 Rab protein (GTPase) present as Rab - GDP GEF / aistory a converted as pab-GTP @ SNARES o 7 vesicle Tott-Snare present iron here V-Snare present ER I cis-golgi I here o budded off @ Rab-GTP Theap V-Snare Cop-I Protein

After going to reach cis-golgi, protein will be die associated and only membrane present that contains v-share and Rab -GTP. Rab-GTP will associate oe binding with Rab effectors which will present at cis-golas → V-snore and t-snare will form bond. They form complex called Share Complex ER complex cis-golgi # Snare complex bind with SNAP & NSF with the help of ATP. The complex gets opened i e; V- SNARE and T-SNARE got Seperated V-SNARE Should be present on ER but it is present on cis-golgi. So, it has to be recycled. That process is called retrograde. cop- V-SNARE will be send ER. to

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