Question

1. DNA Structure and Replication fill in the blanks. 10pts and , have two nitrogenous rings and are a. The bases, called direction and reads the b. DNA Polymerase synthesizes new DNA in the template DNA strand in the direction. c. DNA polymerases use -exonuclease activity to proofread newly synthesized DNA. Whereas, DNA Pol I uses _-exonuclease activity to remove RNA primers. d. The enzyme telomerase synthesizes using as a template. e. DNA Replication begins at the and two ,, creating one 2. Shown below is an mRNA sequence. Design a 15 nucleotide DNA probe to detect the mRNA using nucleic acid hybridization. 4pts. 5' - AAUCGAUGGUCGAAAUGCTCGAUCAGCUAGCUAGC - 3

Answer 1

1. Adenine and guanine and purines.

2. New DNA is made in the 5' to 3' direction. A template reads in 3' to 5' direction.

3. Telomerase is a specialized ribonucleoprotein polymerase that synthesizes the TTAGGG telomeric repeats. The telomerase enzyme uses parts of human telomerase–associated RNA (hTR) as a template for telomeric repeat synthesis.

4. DNA replication begins at the origin of replication to catalyze the formation of one replication bubble with two outward-moving replication forks.

5. A probe is a nucleic acid molecule (single-stranded DNA or RNA) with a strong affinity with a specific target. Probe and target base sequences must be complementary to each other.

So the probe sequence will be TTAGCTACCAGCTTT