Question

Question 6

factors that damage pericytes and lead to increased deposition of ECM material. Because pericytes are related to cells in the kidney that have a sodium-coupled glucose transporter (SGLT), they investigated whether SGLT in retinal pericytes might play a role. Specifically, they examined the link between glucose uptake and collagen synthesis (collagen is a protein found in the basement membrane). The investigators cultured endothelial cells and pericytes separately, then investigated their ability to take up glucose in the presence of Nations, as shown in Figure 1. (A) Glucose uptake (nmol/mo/hr) Q6. Describe and explain the results obtained in Figure 1. a. [SA] Does this experiment support the hypothesis that SGLT exists in pericytes? Explain. Pericytes b. [CA] Is SGLT an example of primary or secondary active transport? 0 20 40 60 80 100 120 140 160 180 Na (mm) F canzoumous for Next, they sought to show that there was a Nat-dependent glucose transporter using Michaelis-Menten kinetics (Figure 2 below). You'll recall from lecture that uptake of molecules by transporters can be examined kinetically in much the same way as enzymes can be studied kinetically. Endothelial cells the 0 20 40 60 80 100 120 140 160 180 Na (mm) Figure 1 - Uptake of Glucose by Cultured Retinal Cells

Answer 1

The graph represents the amount of glucose uptake for synthesis of collagen in the presence of sodium. In the first graph, it is very much clear that the amount of glucose uptake is very much dependent on the concentration of sodium as it increases with increase in sodium concentration. This clearly showsthat SGLT exists in pericytes.

But when we take the case of endothelial cells, it is seen as the glucose uptake is independent of the amount of sodium concentration.  

SGLT is an example for secondaryactive transport