Question

1 of 1 Page 1 1. Consider the following domain diagram of a hypothetical integral membrane protein (black segments regions of nonpolar amino acids). Describe the orientation of this protein in the ER membrane. Where would you expect for find the N and termini? How many transmembrane segments would you predict? + N 2. Consider the partial amino acid sequence of the hypothetical protein shown below. In which cellular compartment would you expect to find the functional protein? Explain your answer NH,-MAVIGLAW .......SGTAEG DEL-COH 3. Describe the activation cycle of the RAN-GTPase. Identify the factor(s) responsible for the directionality of transport across the nuclear envelope. 4. Describe the process of co-translational translocation of a membrane protein from the onset of translation to the initiation of transport across the ER membrane. 5. List all of the cellular compartments in which you would expect to find glycosylated residues of membrane proteins. Name one cellular compartment in which you would not expect to find glycosylated residues of membrane proteins, and explain the reason
1&2 pls

Answer 1

1. As the protein sequence shown in the question is containing 6 regions of the non-polar amino acid residues, it can be clear that for spanning the membrane these regions will be used and thus the membrane will span by the protein 6 times. Thus this transmembrane protein will have 6 turns but as to transfer the signaling the protein will have to take the 6 turns and both N and C-terminals will face towards the cytoplasm of the cell as the protein will be transferred from the cytosol to the extracellular fluid.

2. It has been seen that the sequence provided contains many nonpolar amino acids which state that the peptide sequence is crossing the cell membrane only one as many of them were non-polar and some amino acid residues with the positive charge. And consider the sequence near the C-terminus it can be stated that the negatively charged amino acid residues were also present and thus they will act as the signal transduction element. Hence this amino acid sequence can be present on the cell membrane itself which will detect the ligand to proceed with the cell signal transduction.