Identify the correct operon: Trp/lac/or none.
- has tryptophan but no lactose
- has both tryptophan and lactose
- contains neither.
Has tryptophan but no lactose = Trp
Has both tryptophan and lactose = None
Contains neither = None
Identify the correct operon: Trp/lac/or none. - has tryptophan but no lactose - has both tryptophan...
Which
The figure represents the trp (A-C) and the lac (D-G) operons. A and D show the operons without any proteins bound to them. The others have one or more proteins bound to them. Use the figures your notes to answer the next four questions. OK? = este lace laco acz | lacy = D = trpP tro trpe trpo trpetrpctra EA lacp Lac Rep- pressor lacz lacy RNAP holo enzyme trpo tro trpe trpe treo trpD trpB trpe trc...
a) For the lac operon, will the repressor or RNA polymerase be bound to the operon in this situation? Draw what will be happening on the operon below. PROMOTER OPERATOR Lactose Enzyme 1 Lsctose Enzyme2 Lactose Enzyme 3 NO b) Will transcription occur? c) Describe what is happening (with vocabulary words). YES 2. Bobby Joe is fasting today, how will the E. coli in her stomach respond to the lack of Tryptophan? a) For the trp operon, will the repressor...
Expression of the trp operon is regulated by the level
of free tryptophan in the cell both through repressor action and
attenuation. This chart shows the percent expression of the
trpoperon in the presence and absence of tryptophan for
wild-type (trpR+ or trp+) and repressor mutants
(trpR-).
Use this information to determine the levels of expression for the
following genotypes and conditions.
Rank the genotypes and conditions from highest to lowest level
of trp operon expression.
Please use information below...
In E coli the lac operon will express enzymes for the digestion of (Glucose/Lactose) when it is present, but if both the sugar mentioned before and (Glucose/ Lactose) are present the lac operon is repressed, resulting in the (Glucose/ Lactose) being used first. If there are high levels of cAMP, this means (Glucose/Lactose) levels are low, this results in a (Represser/Promoter) being activated and (Decreasing/Increasing) transcription of the lac operon.
Two mutations of the Trp operon are identified. In mutant A, the operator sequence has changed and the repressor is not able to bind, while as in mutant B the tryptophan is not able to bind the repressor For the phrase below choose the appropriate answer: These cells are able to synthesize tryptophan Mutant A Mutant B Both Mutant A and Mutant B Neither Mutant A nor Mutant B QUESTION 63 Two mutations of the Trp operon are identified. In...
b. What is the polycistron in the Lac operon? In the Trp operon? d. Name an aspect of prokaryotic gene regulation that can only occur in a prokaryote and not a eukaryote e. Provided the wildtype lac operon, give three different examples of mutations, what they target, and how they disrupt WT regulation of the operon. f. Describe how the RNA leader of the Trp operon can repress/induce gene translation
P OPERON What happens to transcription at the trp operon when 1. tryptophan levels are low? Why? 2. Illustrate it. Include: RNA repressors erase, repressors, and any other molecules needed to show how this worke. GENE TURNED ON -+ + +- Promoter OperatorStructural Genes 3. What happens to transcription at the trp operon when trypto 4. Illustrate it. Include: RNA polymerase, e, repressors and any other molecules needed to show the following GENE TURNED OFF Promoter Operator Structural Genes Circle...
ACTIVITY #2: Operon control of gene expressionComplete the comparison of the lac Operon and trp Operons as a means of gene regulation. lac operorn trp operon Regulates production of Name of Regulatory gene" and what it produces What binds to the operator & when does this occur: Structural genes controlled by the operon and how their expression is controlled: High levels of what substance affects the operon how?
For regulation of the trp operon, what happens to in the absence of tryptophan? repressor protein (a) It binds to the operator, and represses transcription of genes in the trp operon (b) It cannot bind to the operator, and transcription of genes in the trp operon takes place (c) It binds to the regulatory gene that codes for the TrpR repressor, and represses its transcription (d) It cannot bind to the regulatory gene that codes for the TrpR repressor, and...
The following activity will give you practice with the two operons we discussed - the lac operon and the trp operon. Below are two scenarios to consider. You will need to determine whether or not transcription is occurring and describe what is happening in the cell using our operon vocabulary words - regulatory gene, RNA polymerase, operator, promoter, repressor, and genes. 1. Bobby Joe just enjoyed an In and Out milkshake, how will the E. coli in her stomach respond?...