Question

1) You are studying two different forms of an enzyme. One form is from the wildtype allele. The other is a mutant version. A partial sequence of the wildtype allele is shown here. The underlined area is of particular interest to you, since it includes where the mutation may occur. Design a set of 10nt primers that will successfully amplify this region. Be sure to write the primers in the correct orientation, as is typical for molecular biologists. a. forward primer sequence: b. reverse primer sequence: Template DNA CCACCATGAA TATTGTACGG TACCATAAAT ACTTGACCAC CTGTAGTACA TAAAAACCCA ATCCACATCA AAACCCCCTC 20 CCCATGCTTA CAAGCAAGTA CAGCAATCAA CCCTGAATTC TCACACATCA ACTGCAATTC CAAAGCCACC сстCACсCAC od TAGGAGACCA ACAAACCTAC ССАСccТТАА CAGTACATAG TACATAAAGC CATTTACCGT ACATAGCACA TTACAGTCAA wanaume mms Internationer ATCCCTTCTC GTCCCCATGG ATGACCCCCC TCAGATAGGG OG UTIVE 103 mm 1 l but how to w brobballo e luogo dira od 10 r ung Goodwin gioco noso 1 .1 Dan 1

Answer 1

Answer:

Forward primer: 5' ATGTATTTTT 3'

Forward primer should be complementary to the starting 10 bases of the sequence we want to amplify.

Reverse primer: 5' TGTACTGTTA 3'

Reverse primer should be reverse complimentary to the end 10 bases of the sequence we want to amplify.