Which will happen when Lys140 (this Lys forms ionic bond with His 146) is mutated to...
Which will happen when Lys140 (this Lys forms ionic bond with His 146) is mutated to threonine in the beta globin chain of hemoglobin?
A. 2,3 BPG will bind tightly
B. 2,3 BPG will bind less tightly
C. O2 affinity of hemoglobin will be reduced
D. O2 affinity of hemoglobin will be increased
E. Both B and D
Homework Answers
O2 affinity of haemoglobin will be increased. Beacsue subsitution of lys with Thr cause the disruption in H-bonding and destabilize the T state , so reduce the affinity for 2,3BPG and R state will be more stable hence O2 affinity will be enhanced.
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