Question

Show your work and share your logic with respect to what reactions use either ATP of reduced electron carriers and what reactions produce either ATP or reduced electron carriers. Then show the final NET total of ATP's produced for that molecule as well as the average number of ATP/Carbon of the original molecule. Note that I am asking you to think through total oxidation of the fatty acid to CO2. 1. Calculate the net ATPs obtained with a 18:149 fatty acid with a single branch at carbon 3. SHOW ALL OF YOUR WORK.

Answer 1

oleate is a monounsaturated fatty acid having single double bond. in the 1st step oleate is converted to oleoyl coA like other saturated fatty acid then enters the carnetine shuttle where it undergoes oxidation to yeild 3 acetyl coA molecule and cis dodecenolycoA but as this cannot act as a substrate for enoy coA hydratase to act upon so an enzyme enoyl coA isomerase converts it to transdedecenoyl coA. NOw the enoylcoA hydratase enzyme acts upon it and converts into beta HydroxyacylcoA which is then futher oxidised by normal b oxidation process to 6acetyl coA molecule.now all togather 9 acetylcoA molecule are produced from 1 monounsaturated fatty acid.(oleic acid)

S.COM ß Onidation three eyell) (73 Acelye of Oleoyl-CoA 'S-CoA ris. - do decencyl- COA enoyl con isomerase -s-WA trans z dodecenoye wa halose emagle con hydratose noul to é G-CoA Bondation (5 eyele) u .6 Acelyl-CoA so net ATP obtained is 8 NADH (2.5 ATP / NADH) sc, 8 x 2.5 = 20 ATP I FADH (15 ATP) RADH) sc, 7715 - 20'5 ATP a acetyl wA (10 ATP) so, 9+10 = 90 ATP - 120.5 ATP moleance 2 ATP molecule are - 2.0 ATP required for achivalion so 118.5 ATP molecule (-2) ATP molecule