Picture is at bottom. The figure of the western blot. Figure
legend is also below that. 
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Picture is at bottom. The figure of the western blot. Figure
legend is also below that....
31. FGF receptor 1 (FGFR1) belongs to a family of tyrosine kinase receptors often amplified in...
31. FGF receptor 1 (FGFR1)
belongs to a family of tyrosine kinase receptors often amplified in
lung cancer. H520 cell line was stimulated by bFGF (20
ng/ml) for 20 min, with or without the pretreatment of AZD4547 (1
μM) for 40min. Then the cells were collected and analyzed by
Western blot (figure A). H520 cells were also stimulated
with bEGF (20 ng/ml) and protein levels were analyzed after 1, 6,
12, and 24 hours after bEGF treatment (Figure
B). Lastly, mRNA levels of...
1. The figure below showsaWestern blot analysis of protein extractsfrom cells (starved for 16 hours) untreated...
1. The figure below showsaWestern blot analysis of protein extractsfrom cells (starved for 16 hours) untreated or Epidermal Growth Factor EGF-treated (100 ng/ml), using Phospho-mTOR (Ser2448) Antibody (upper) or control mTOR Antibody (lower). What is the main conclusion that can be reached from this analysis?
I need help with this question below. Researchers generated a transgenic strain of mice called DrpTg...
I need help with this question below. Researchers generated a
transgenic strain of mice called DrpTg which overexpresses a
protein called Drp1 under a muscle-specific promoter. DrpTg mice
had poor postnatal weight gain, smaller muscle mass and lower
muscle strength than WT mice of the same age and fed the same diet.
The experiments shown in the left panel below showed that
phosphorylation of the normal cellular protein labeled P-? was
increased in DrpTG muscle compared to WT muscle tissue....
I need help with a and b please. 4) Based on the image below (from Dean...
I need help with a and b please.
4) Based on the image below (from Dean et al 2001), answer the following questions (7). Serine/threonine protein phosphatase 5 (PP5) Control Nuclear > Cytoplasmic Localization of GR-GFP (%) ISIS 18321 Dermethasone Control Dexamethasone Dexamethasone Figure 2: Effects of ISIS 15534 and dexamethasone on the subcellular distribution of GR-GFP. A. Representative subcellular distribution of GR-GFP. A549 cells were grown and treated with ISIS 15521 (a mismatched control antisense oligonucleotide for ISIS 1535),...
Parkin Mannosidase II Merge Parkin Y-Adaptin Merge Parkin BIP Merge Parkin LAMP-1 Merge Parkin Ubiquitin Merge...
Parkin Mannosidase II Merge Parkin Y-Adaptin Merge Parkin BIP Merge Parkin LAMP-1 Merge Parkin Ubiquitin Merge FIG. 2. Immunocytochemical detection of overexpressed Par- kin. COS-7 cells were transfected with FLAG-Parkin and subjected to co-immunostaining with anti-FLAG antibody (green) along with the Golgi complex marker mannosidase II (A), trans-Golgi network marker yadaptin (B), endoplasmic reticulum marker BIP/GRP78 (C), and ly- sosomal marker LAMP-1 (D), all with red fluorescence. E, COS-7 cells transfected with FLAG-Parkin were immunostained with anti-FLAG antibody (green) and...
Please explain figures 6C, 6D, 6F, 6E, and 6G in simple terms! I am having difficulty...
Please explain figures 6C, 6D, 6F, 6E, and 6G in simple terms! I
am having difficulty understanding the figures. I have copy and
pasted a section in the article. Thank you!!
Figure 6. MLL5 Suppresses Differentiation of GBM
Self-Renewing Cells
(A) Western blot to assess the effects of MLL5 knockdown in the
GBM primary culture G432NS.
(B) Western blots on cell lysates from two GBM cultures
differentiated over a 21-day period.
(C) Heatmap and unsupervised hierarchical clustering of
differentially regulated...
Please explain figures 6C, 6D, 6F, and 6G in simple terms! I am having difficulty understanding...
Please explain figures 6C, 6D, 6F, and 6G in simple
terms! I am having difficulty understanding the figures. I have
copy and pasted a section in the article. Thank you!!
Figure 6. MLL5 Suppresses Differentiation of GBM
Self-Renewing Cells
(A) Western blot to assess the effects of MLL5 knockdown in the
GBM primary culture G432NS.
(B) Western blots on cell lysates from two GBM cultures
differentiated over a 21-day period.
(C) Heatmap and unsupervised hierarchical clustering of
differentially regulated genes...
Look at the figure below, borrowed from Chao et al. (2007), "MeCP2 Controls Excitatory Synaptic Strength...
Look at the figure below, borrowed from Chao et al. (2007), "MeCP2 Controls Excitatory Synaptic Strength by Regulating Glutamatergic Synapse Number." In this experiment, individual neurons were grown in culture such that they only formed synapses on themselves (autapses). Three proteins were visualized (VGLUT1, MAP2, PSD95), under three different conditions: wildtype neurons (WT), MeCP2 knockout neurons (Null), and MeCP2 overexpressing neurons (Tg1). A VOLUT1 MAP2B PSD95 C VGLUT1 MAP2 PSD95 MAP2 DIV 7-9 *** 2.0, T WT ** --- *HH...
Can you please explain in simple terms the methods of this experiment? I'm having difficulty visualizing...
Can you please explain in simple terms the methods of this experiment? I'm having difficulty visualizing and understanding what is being done. I have copied and pasted some of the text from the article. Thank you! Abstract : Obesity, high-fat diets, and subsequent type 2 diabetes (T2DM) are associated with cognitive impairment. Moreover, T2DM increases the risk of Alzheimer's disease (AD) and leads to abnormal elevation of brain beta-amyloid levels, one of the hallmarks of AD. The psychoactive alkaloid caffeine...
Identify the scientific premise, main hypothesis addressed by the paper and the research question of the...
Identify the scientific premise, main hypothesis addressed by
the paper and the research question of the paper
Also, what is the main results and the implication of
results
IMPORTANCE Clinical outcomes for glioblastoma remain poor. Treatment with immune checkpoint blockade has shown benefits in many cancer types. To our knowledge, data from a randomized phase 3 clinical trial evaluating a programmed death-1 (PD-1) inhibitor therapy for glioblastoma have not been reported. OBJECTIVE To determine whether single-agent PD-1 blockade with nivolumab...
31. FGF receptor 1 (FGFR1)
belongs to a family of tyrosine kinase receptors often amplified in
lung cancer. H520 cell line was stimulated by bFGF (20
ng/ml) for 20 min, with or without the pretreatment of AZD4547 (1
μM) for 40min. Then the cells were collected and analyzed by
Western blot (figure A). H520 cells were also stimulated
with bEGF (20 ng/ml) and protein levels were analyzed after 1, 6,
12, and 24 hours after bEGF treatment (Figure
B). Lastly, mRNA levels of...
I need help with this question below. Researchers generated a
transgenic strain of mice called DrpTg which overexpresses a
protein called Drp1 under a muscle-specific promoter. DrpTg mice
had poor postnatal weight gain, smaller muscle mass and lower
muscle strength than WT mice of the same age and fed the same diet.
The experiments shown in the left panel below showed that
phosphorylation of the normal cellular protein labeled P-? was
increased in DrpTG muscle compared to WT muscle tissue....
I need help with a and b please.
4) Based on the image below (from Dean et al 2001), answer the following questions (7). Serine/threonine protein phosphatase 5 (PP5) Control Nuclear > Cytoplasmic Localization of GR-GFP (%) ISIS 18321 Dermethasone Control Dexamethasone Dexamethasone Figure 2: Effects of ISIS 15534 and dexamethasone on the subcellular distribution of GR-GFP. A. Representative subcellular distribution of GR-GFP. A549 cells were grown and treated with ISIS 15521 (a mismatched control antisense oligonucleotide for ISIS 1535),...
Parkin Mannosidase II Merge Parkin Y-Adaptin Merge Parkin BIP Merge Parkin LAMP-1 Merge Parkin Ubiquitin Merge FIG. 2. Immunocytochemical detection of overexpressed Par- kin. COS-7 cells were transfected with FLAG-Parkin and subjected to co-immunostaining with anti-FLAG antibody (green) along with the Golgi complex marker mannosidase II (A), trans-Golgi network marker yadaptin (B), endoplasmic reticulum marker BIP/GRP78 (C), and ly- sosomal marker LAMP-1 (D), all with red fluorescence. E, COS-7 cells transfected with FLAG-Parkin were immunostained with anti-FLAG antibody (green) and...
Please explain figures 6C, 6D, 6F, 6E, and 6G in simple terms! I
am having difficulty understanding the figures. I have copy and
pasted a section in the article. Thank you!!
Figure 6. MLL5 Suppresses Differentiation of GBM
Self-Renewing Cells
(A) Western blot to assess the effects of MLL5 knockdown in the
GBM primary culture G432NS.
(B) Western blots on cell lysates from two GBM cultures
differentiated over a 21-day period.
(C) Heatmap and unsupervised hierarchical clustering of
differentially regulated...
Please explain figures 6C, 6D, 6F, and 6G in simple
terms! I am having difficulty understanding the figures. I have
copy and pasted a section in the article. Thank you!!
Figure 6. MLL5 Suppresses Differentiation of GBM
Self-Renewing Cells
(A) Western blot to assess the effects of MLL5 knockdown in the
GBM primary culture G432NS.
(B) Western blots on cell lysates from two GBM cultures
differentiated over a 21-day period.
(C) Heatmap and unsupervised hierarchical clustering of
differentially regulated genes...
Look at the figure below, borrowed from Chao et al. (2007), "MeCP2 Controls Excitatory Synaptic Strength by Regulating Glutamatergic Synapse Number." In this experiment, individual neurons were grown in culture such that they only formed synapses on themselves (autapses). Three proteins were visualized (VGLUT1, MAP2, PSD95), under three different conditions: wildtype neurons (WT), MeCP2 knockout neurons (Null), and MeCP2 overexpressing neurons (Tg1). A VOLUT1 MAP2B PSD95 C VGLUT1 MAP2 PSD95 MAP2 DIV 7-9 *** 2.0, T WT ** --- *HH...
Identify the scientific premise, main hypothesis addressed by
the paper and the research question of the paper
Also, what is the main results and the implication of
results
IMPORTANCE Clinical outcomes for glioblastoma remain poor. Treatment with immune checkpoint blockade has shown benefits in many cancer types. To our knowledge, data from a randomized phase 3 clinical trial evaluating a programmed death-1 (PD-1) inhibitor therapy for glioblastoma have not been reported. OBJECTIVE To determine whether single-agent PD-1 blockade with nivolumab...
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