If you were setting up a 20μl restriction digest, how much of the 5X buffer would...

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If you were setting up a 20μl restriction digest, how much of the 5X buffer would...

If you were setting up a 20μl restriction digest, how much of the 5X buffer would you use?

	a.	2.0 μl
	b.	4.0 μl
	c.	16 μl
	d.	5 times the volume of restriction endonuclease used.

science biology

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Answer 1

Answer #1

4 ul of the reaction buffer can be used. The concentration of the buffer in the final volume should be 1X. To make that, 4 ul of the 5x reaction buffer can be used. Generally, we follow this criteria.

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Answer 2

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