In lane 3, top most band from loading point (sample loading side) is linear as after digestion with restriction enzyme it's become linear so occupied more surface area and hence slow running.
Below than linear band, is band of nick one in which it's one strand is relaxed while other is supercoiled so occiped average surface area due to partial digestion of plasmid due to restriction enzyme.
While lower most band is of supercoiled as plasmid is still circular and hence surface area is also less so running fast.
Hope it's clear .thanks
in regards to lane 3 of this gel, which band is nicked, linear and supercoiled? Lysis...
I need the answers for questions 2 and 3. My DNA ladder is in lane 2 marked by the yellow arrow. Thanks! Here is the only other info I have. Thanks! Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....