Question

What disadvantages do physical methods have when compared to biological transfer methods? (discussing gene-transfer methods)

Answer 1

The disadvantages of physical methods when compared to biological transfer methods, are as follows:

physical method disadvantages

1. Microinjection is the equipment which is very refined and expensive, the operating techniques require long hours of practice, and only limited cells can be injected at a time.
2. Gene gun requires costly devices and reagents such as gold particles. and gene guns evoke high humoral immune responses.
3. Ultrasound has disadvantages such as short-range of gene transfer, the necessity of a surgical procedure, and tissue damage. Distance between electrodes normally requires 1 cm and is not good for a large area.

while beside these biological gene transfer has benefits like high frequency of gene transfer, transfer into specific cell types, more control over the final copy number of a transferred gene, enable them to express foreign genes.

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