What is the advantage of using microarray analysis to study gene expression differences when compared to...
What is the advantage of using microarray analysis to study gene expression differences when compared to RT-PCR or Northern analysis?
Homework Answers
The biggest advantage of microarray technology over RT-PCR or Northern analysis is to offer is a large number of transcripts that can be quantified in a single experiment. DNA microarrays has the capability to meaure tens of thousands of gene expression simultaneously.
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What is the advantage of using microarray analysis to study gene
expression differences when compared to...
Tissue-specific microarray analysis facilitates the comparison of gene expression in different tissues. In order to prepare...
Tissue-specific microarray analysis facilitates the comparison
of gene expression in different tissues. In order to prepare a
sample for microarray analysis, messenger RNA (mRNA) is extracted
from a particular tissue, reverse transcribed to generate
complementary DNA (cDNA), and labeled with a fluorescent probe.
The microarray below shows comparison of gene expression in
stomach and intestinal tissues. The cDNA from stomach tissue was
labeled with red fluorescent probes, whereas the cDNA from the
intestinal tissue was labeled with green fluorescent probes....
Northern blotting, RT-PCR, and microarrays can be used to analyze gene expression. A lab studies yeast...
Northern blotting, RT-PCR, and microarrays can be used to analyze gene expression. A lab studies yeast cells, comparing their growth in two different sugars, glucose and galactose. One student is comparing expression of the gene HMG2 under these two conditions. Which technique(s) could he use and why? Another student wants to compare expression of all the genes on chromosome 4, of which there are approximately 800. What technique(s) could she use and why?
Question 4 2 pts What is the main advantage of the CRISPRCas method over the RT-PCR...
Question 4 2 pts What is the main advantage of the CRISPRCas method over the RT-PCR method? CRISPR is more accurate than PCR CRISPR detects individuals that are sick and individuals that had the disease but are not sick anymore. The test can be performed in less than 1 hr where the sample was taken. CRISPR is less expensive than PCR Question 5 4 pts What are some differences between the PCR that occurs in the Sherlock protocol and the...
check all that apply What are some differences between the PCR that occurs in the Sherlock...
check all that apply
What are some differences between the PCR that occurs in the Sherlock protocol and the PCR that occurs in RT-PCR test? O PCR in Sherlock protocol can amplify more than one gene at the same time O Dyes are added to RT-PCR PCR in RT-PCR is faster PCR in Sherlock protocol is isothermic (all the steps occur at the same temperature).
1. Bacteroides thetaiotamicron is an important member of the gut microbiome. Recent experiments showed that gene...
1. Bacteroides thetaiotamicron is an important member of the gut microbiome. Recent experiments showed that gene expression in this organism within the mouse microbiome could be modulated precisely using a. RT-PCR. b. CRISPR-Cas9. c. SDS-PAGE. d. BLAST. 2. A reporter gene a. allows for Southern and Northern blots to be probed. b. can be assayed readily through its activity. c. is a regulatory gene that activates other genes. d. is a mutant gene that identifies the function through its defects.
Gene expression analysis is being done when studying what Metabolomics Transcriptomics Genomics Nutriogenetics
Gene expression analysis is being done when studying what Metabolomics Transcriptomics Genomics Nutriogenetics
Which of the following is the most appropriate indication of gene expression level? A ONA 6....
Which of the following is the most appropriate indication of gene expression level? A ONA 6. Protein CRNA DmRNA QUESTION 14 In reality, what is being directly quantified in real-time rt-PCR to estimate the gene expression level? OM Protein levels by amplifying with ribosomes using fluorescently tagged amino acids B. DNA which is amplified with gene-specific primers and measured for fluorescence Reverse transcriptase levels which indicates rate of mRNA transcription O mRNA which is amplified with gene-specific primers and measured...
what are the big differences in the way communities approach development today compared to 1990, when...
what are the big differences in the way communities approach development today compared to 1990, when porter wrote the competitive advantage of nations
Gene expression analysis is being done when studying …. Metabolomics Transcriptomics Genomics Nutriogenetics
Gene expression analysis is being done when studying …. Metabolomics Transcriptomics Genomics Nutriogenetics
If you want to measure gene expression using PCR, why do you have to use cDNA...
If you want to measure gene expression using PCR, why do you have to use cDNA and not gDNA?
Tissue-specific microarray analysis facilitates the comparison
of gene expression in different tissues. In order to prepare a
sample for microarray analysis, messenger RNA (mRNA) is extracted
from a particular tissue, reverse transcribed to generate
complementary DNA (cDNA), and labeled with a fluorescent probe.
The microarray below shows comparison of gene expression in
stomach and intestinal tissues. The cDNA from stomach tissue was
labeled with red fluorescent probes, whereas the cDNA from the
intestinal tissue was labeled with green fluorescent probes....
Question 4 2 pts What is the main advantage of the CRISPRCas method over the RT-PCR method? CRISPR is more accurate than PCR CRISPR detects individuals that are sick and individuals that had the disease but are not sick anymore. The test can be performed in less than 1 hr where the sample was taken. CRISPR is less expensive than PCR Question 5 4 pts What are some differences between the PCR that occurs in the Sherlock protocol and the...
check all that apply
What are some differences between the PCR that occurs in the Sherlock protocol and the PCR that occurs in RT-PCR test? O PCR in Sherlock protocol can amplify more than one gene at the same time O Dyes are added to RT-PCR PCR in RT-PCR is faster PCR in Sherlock protocol is isothermic (all the steps occur at the same temperature).
Which of the following is the most appropriate indication of gene expression level? A ONA 6. Protein CRNA DmRNA QUESTION 14 In reality, what is being directly quantified in real-time rt-PCR to estimate the gene expression level? OM Protein levels by amplifying with ribosomes using fluorescently tagged amino acids B. DNA which is amplified with gene-specific primers and measured for fluorescence Reverse transcriptase levels which indicates rate of mRNA transcription O mRNA which is amplified with gene-specific primers and measured...
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