Gene expression analysis is being done when studying ….
Metabolomics
Transcriptomics
Genomics
Nutriogenetics
Gene expression analysis is being done when studying Genomics
Genomics is the new science that deals with the discovery and noting of all the sequences in the entire genome of a particular organism. The genome can be defined as the complete set of genes inside a cell. Genomics, is, therefore, the study of the genetic make-up of organisms.
Determining the genomic sequence, however, is only the beginning of genomics. Once this is done, the genomic sequence is used to study the function of the numerous genes (functional genomics), to compare the genes in one organism with those of another (comparative genomics), or to generate the 3-D structure of one or more proteins from each protein family, thus offering clues to their function (structural genomics).
In crop agriculture, the main purpose of the application of genomics is to gain a better understanding of the whole genome of plants. Agronomically important genes may be identified and targeted to produce more nutritious and safe food while at the same time preserving the environment.
Genomics is an entry point for looking at the other ‘omics’ sciences. The information in the genes of an organism, its genotype, is largely responsible for the final physical makeup of the organism, referred to as the “phenotype”. However, the environment also has some influence on the phenotype.
DNA in the genome is only one aspect of the complex mechanism that keeps an organism running – so decoding the DNA is one step towards understanding the process. However, by itself, it does not specify everything that happens within the organism.
The basic flow of genetic information in a cell is as follows. The DNA is transcribed or copied into a form known as “RNA”. The complete set of RNA (also known as its transcriptome) is subject to some editing (cutting and pasting) to become messenger-RNA, which carries information to the ribosome, the protein factory of the cell, which then translates the message into protein.
Gene expression analysis is being done when studying …. Metabolomics Transcriptomics Genomics Nutriogenetics
Gene expression analysis is being done when studying what Metabolomics Transcriptomics Genomics Nutriogenetics
Functional Genomics is Comparing the genome of different individuals Studying the evolution of the human genome Determining the role of all genes through gene disruption Labelling the ancient parts of the genome
What is the advantage of using microarray analysis to study gene expression differences when compared to RT-PCR or Northern analysis?
Someone explain how use of genomics and co-expression analysis can be used to generate hypotheses regarding the biosynthesis of secondary plant cell wall polymers such as xylans and lignin and how these hypotheses may be tested using reverse genetics approaches.
Suppose scientists studying pigmentation in Arabidopsis attempted to alter expression of a pigment gene. Using transgenic techniques, multiple copies of the pigment gene were inserted into homozygous and heterozygous individuals. This was expected to darken the pigmentation of the plants. Instead of increased pigment production, the additional gene copies often reduced or completely inhibited the pigment gene expression. One explanation for this unexpected result is that high concentrations of mRNA are produced. This, in turn, increases the likelihood of homologous...
Eric is studying the evolution of species. After performing DNA analysis, he discovers a gene that is conserved from yeast to humans. In which cellular processes is this gene most like to be involved? Could this gene have evolved to confer some of the complex traits that we observe in modern animals, like multicellularity? Briefly explain.
Trivia Questions 1. This level of control in gene expression prevents a gene from being transcribed when it is not needed. 2. These genes produce proteins that govern the activity of structural genes. 3. These intervening sequences are cut out of an mRNA transcript before it can leave the nucleus. 4. A chromosome is made up of these two molecules. 5. This term refers to DNA that is composed of at least two species. 6. These two vectors are used...
This is the gene expression analysis of actin and EGFR in breast cancer What do the images represent? Describe axis. What can you tell about the importance of actin and EGFR genes in breast cancer?
6. The three types of RNA involved in gene expression are mRNA tRNA complimentary strand of RNA during gene expression is this strand of RNA is being synthesized, each new nucleotide is being added to the strand via a bond formed at carbon number the nucleotide at the end of the RNA. The enzyme invoved involved in transcribing a When , and rRNA of the ribose ring present on
Tissue-specific microarray analysis facilitates the comparison
of gene expression in different tissues. In order to prepare a
sample for microarray analysis, messenger RNA (mRNA) is extracted
from a particular tissue, reverse transcribed to generate
complementary DNA (cDNA), and labeled with a fluorescent probe.
The microarray below shows comparison of gene expression in
stomach and intestinal tissues. The cDNA from stomach tissue was
labeled with red fluorescent probes, whereas the cDNA from the
intestinal tissue was labeled with green fluorescent probes....